Literature DB >> 28510362

Burkholderia thailandensis: Genetic Manipulation.

Erin C Garcia1.   

Abstract

Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis. © 2017 by John Wiley & Sons, Inc.
Copyright © 2017 John Wiley & Sons, Inc.

Entities:  

Keywords:  allelic exchange; conjugation; mini-Tn7; mutation; transformation Burkholderia thailandensis

Mesh：

Year:  2017        PMID： 28510362      PMCID： PMC5434709          DOI： 10.1002/cpmc.27

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  22 in total

1.  Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading.

Authors:  W Kespichayawattana; S Rattanachetkul; T Wanun; P Utaisincharoen; S Sirisinha
Journal:  Infect Immun       Date:  2000-09       Impact factor: 3.441

2.  Dissection of the Burkholderia intracellular life cycle using a photothermal nanoblade.

Authors:  Christopher T French; Isabelle J Toesca; Ting-Hsiang Wu; Tara Teslaa; Shannon M Beaty; Wayne Wong; Minghsun Liu; Imke Schröder; Pei-Yu Chiou; Michael A Teitell; Jeff F Miller
Journal:  Proc Natl Acad Sci U S A       Date:  2011-07-05       Impact factor: 11.205

3.  Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome.

Authors:  G Pósfai; V Kolisnychenko; Z Bereczki; F R Blattner
Journal:  Nucleic Acids Res       Date:  1999-11-15       Impact factor: 16.971

Review 4.  Tn7.

Authors:  Joseph E Peters
Journal:  Microbiol Spectr       Date:  2014-10

5.  Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.

Authors:  Michael H Norris; Yun Kang; Bruce Wilcox; Tung T Hoang
Journal:  Appl Environ Microbiol       Date:  2010-09-17       Impact factor: 4.792

6.  Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei.

Authors:  Kyoung-Hee Choi; Takehiko Mima; Yveth Casart; Drew Rholl; Ayush Kumar; Ifor R Beacham; Herbert P Schweizer
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

7.  Burkholderia BcpA mediates biofilm formation independently of interbacterial contact-dependent growth inhibition.

Authors:  Erin C Garcia; Melissa S Anderson; Jon A Hagar; Peggy A Cotter
Journal:  Mol Microbiol       Date:  2013-08-16       Impact factor: 3.501

8.  Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation.

Authors:  Josephine R Chandler; Breck A Duerkop; Aaron Hinz; T Eoin West; Jake P Herman; Mair E A Churchill; Shawn J Skerrett; E Peter Greenberg
Journal:  J Bacteriol       Date:  2009-07-31       Impact factor: 3.490

9.  Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments.

Authors:  Metawee Thongdee; Larry A Gallagher; Mark Schell; Tararaj Dharakul; Sirirurg Songsivilai; Colin Manoil
Journal:  Appl Environ Microbiol       Date:  2008-02-29       Impact factor: 4.792

10.  Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.

Authors:  Joanne M Stevens; Ricky L Ulrich; Lowrie A Taylor; Michael W Wood; David Deshazer; Mark P Stevens; Edouard E Galyov
Journal:  J Bacteriol       Date:  2005-11       Impact factor: 3.490
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  4 in total

1.  An EmrB multidrug efflux pump in Burkholderia thailandensis with unexpected roles in antibiotic resistance.

Authors:  Afsana Sabrin; Brennan W Gioe; Ashish Gupta; Anne Grove
Journal:  J Biol Chem       Date:  2018-12-13       Impact factor: 5.157

2.  A c-di-GMP Signaling Cascade Controls Motility, Biofilm Formation, and Virulence in Burkholderia thailandensis.

Authors:  Zhuo Wang; Xiaorong Xie; Daohan Shang; Laigong Xie; Yueyue Hua; Li Song; Yantao Yang; Yao Wang; Xihui Shen; Lei Zhang
Journal:  Appl Environ Microbiol       Date:  2022-03-24       Impact factor: 5.005

3.  A Burkholderia thailandensis DedA Family Membrane Protein Is Required for Proton Motive Force Dependent Lipid A Modification.

Authors:  Pradip R Panta; William T Doerrler
Journal:  Front Microbiol       Date:  2021-01-12       Impact factor: 5.640

4.  Characterization of a Type VI Secretion System vgrG2 Gene in the Pathogenicity of Burkholderia thailandensis BPM.

Authors:  Jin Li; Wei-Wei Hu; Guo-Xin Qu; Xiao-Rong Li; Yi Xiang; Peng Jiang; Jiang-Qiao Luo; Wen-Huan He; Yu-Jia Jin; Qiong Shi
Journal:  Front Microbiol       Date:  2022-01-05       Impact factor: 5.640
  4 in total

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