Narges Zabihi1, Samira Sadeghi2, Hossein Tabatabaeian2, Kamran Ghaedi3, Mansoureh Azadeh4, Mohammad Fazilati1. 1. Department of Biochemistry, Payame Noor University of Isfahan, Isfahan, Iran. 2. Department of Biology, Division of Genetics, Faculty of Sciences, University of Isfahan, Isfahan, Iran. 3. Department of Biology, Division of Cellular and Molecular Biology, Faculty of Sciences, University of Isfahan; Department of Cellular Biotechnology, Cell Science Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. 4. Zistfanavari Novin Biotechnology Institute, Isfahan, Iran.
Abstract
CONTEXT: A number of single nucleotide polymorphisms (SNPs) in ERBB4 gene have been linked to increase the risk of breast cancer. However, no study has been dedicated to analyze the significance of microRNA-related SNP rs1972820, located in ERBB4 3'-untranslated region (UTR), in breast tumors. AIMS: Here, we investigated the frequency and association between rs1972820 and breast cancer. SUBJECTS AND METHODS: The rs1972820 genotypes in 182 samples were collected from 96 healthy people, and 86 breast cancer patients were determined using tetra-primer amplification refractory mutation system-polymerase chain reaction. The frequency of genotypes was analyzed to find the association between rs1972820 and breast cancer risk. STATISTICAL ANALYSIS USED: Conditional logistic regression, odds ratios (ORs), the associated 95% confidence intervals (CIs), and Armitage's test were used in this study. RESULTS: In silico analysis suggested that rs1972820 located in the 3'UTR of ERBB4 gene affects the binding affinity of miR-3144-3p a potential oncomiRNA. Statistical analysis showed a significant association between SNP rs1972820 G allele and reduced breast cancer risk, odds ratio = 0.443 (95% CI: 0.196-0.998). CONCLUSIONS: rs1972820 SNP allele is significantly associated with the reduced risk of breast cancer and could be considered as a potential marker for breast cancer predisposition in population of Isfahan.
CONTEXT: A number of single nucleotide polymorphisms (SNPs) in ERBB4 gene have been linked to increase the risk of breast cancer. However, no study has been dedicated to analyze the significance of microRNA-related SNP rs1972820, located in ERBB4 3'-untranslated region (UTR), in breast tumors. AIMS: Here, we investigated the frequency and association between rs1972820 and breast cancer. SUBJECTS AND METHODS: The rs1972820 genotypes in 182 samples were collected from 96 healthy people, and 86 breast cancerpatients were determined using tetra-primer amplification refractory mutation system-polymerase chain reaction. The frequency of genotypes was analyzed to find the association between rs1972820 and breast cancer risk. STATISTICAL ANALYSIS USED: Conditional logistic regression, odds ratios (ORs), the associated 95% confidence intervals (CIs), and Armitage's test were used in this study. RESULTS: In silico analysis suggested that rs1972820 located in the 3'UTR of ERBB4 gene affects the binding affinity of miR-3144-3p a potential oncomiRNA. Statistical analysis showed a significant association between SNP rs1972820 G allele and reduced breast cancer risk, odds ratio = 0.443 (95% CI: 0.196-0.998). CONCLUSIONS:rs1972820 SNP allele is significantly associated with the reduced risk of breast cancer and could be considered as a potential marker for breast cancer predisposition in population of Isfahan.