| Literature DB >> 28508217 |
Christopher C Stobart1,2, Anne L Hotard1,2, Jia Meng1,2, Martin L Moore3,4.
Abstract
Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.Entities:
Keywords: BAC; Bacterial artificial chromosome; RSV; Respiratory syncytial virus; Reverse genetics; Virus recovery
Mesh：
Year: 2017 PMID： 28508217 DOI： 10.1007/978-1-4939-6964-7_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745