Zongrong Gong1, Min Shu1, Qing Xia2, Shan Tan1, Wei Zhou3, Yu Zhu1, Chaomin Wan4. 1. Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041,PR China, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education (Sichuan University). 2. Department of Pediatrics, the People' s Hospital of Aba, Maerkang 624000, PR China. 3. Department of Clinical Laboratory, West China Second Hospital, Sichuan University, Chengdu 610041, PR China. 4. Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041,PR China, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education (Sichuan University). wcm0220@126.com.
Abstract
BACKGROUND/AIM: To describe the epidemiological profile of nasal carriage of Staphylococcus aureus (S. aureus) strains, its antibiotic resistance and mecA and Panton Valentine leukocidin (PVL) genes presence, in school children residing in high altitude areas of Southwestern China. METHODS: The cross sectional study screened nasal swabs taken from students for S.aureus. PCR was performed to identify mecA and PVL genes. RESULTS: Of the total 314 children 5.10% (16/314) was detected S.aureus. The resistance of isolated strains to penicillin, erythromycin, clindamycin, rifampicin and cefoxitin was 100%, 81.3%, 81.3%, 0.0%, and 6.3% respectively. No strains demonstrated resistance to vancomycin; expression of mecA gene was detected in 3 isolates and 10 isolates were PVL-positive. CONCLUSION: S. aureus was detected in 5.10% (16/314) of the study population; 0.96% (3/314) had methicillin resistant S.aureus (MRSA); expression of the mecA and PVL genes were detected in 3 and 10 isolates respectively.
BACKGROUND/AIM: To describe the epidemiological profile of nasal carriage of Staphylococcus aureus (S. aureus) strains, its antibiotic resistance and mecA and Panton Valentine leukocidin (PVL) genes presence, in school children residing in high altitude areas of Southwestern China. METHODS: The cross sectional study screened nasal swabs taken from students for S.aureus. PCR was performed to identify mecA and PVL genes. RESULTS: Of the total 314 children 5.10% (16/314) was detected S.aureus. The resistance of isolated strains to penicillin, erythromycin, clindamycin, rifampicin and cefoxitin was 100%, 81.3%, 81.3%, 0.0%, and 6.3% respectively. No strains demonstrated resistance to vancomycin; expression of mecA gene was detected in 3 isolates and 10 isolates were PVL-positive. CONCLUSION:S. aureus was detected in 5.10% (16/314) of the study population; 0.96% (3/314) had methicillin resistant S.aureus (MRSA); expression of the mecA and PVL genes were detected in 3 and 10 isolates respectively.