Literature DB >> 28504294

Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method.

Kai Zhang1, Ke Wang, Xue Zhu, Minhao Xie.   

Abstract

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity.

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Year:  2017        PMID： 28504294     DOI： 10.1039/c7cc02231j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  2 in total

1.  Determination of the activity of uracil-DNA glycosylase by using two-tailed reverse transcription PCR and gold nanoparticle-mediated silver nanocluster fluorescence: a new method for gene therapy-related enzyme detection.

Authors:  Kai Zhang; Wanting Huang; Yue Huang; Ke Wang; Xue Zhu; Minhao Xie
Journal:  Mikrochim Acta       Date:  2019-02-15       Impact factor: 5.833

2.  A strategy combining 3D-DNA Walker and CRISPR-Cas12a trans-cleavage activity applied to MXene based electrochemiluminescent sensor for SARS-CoV-2 RdRp gene detection.

Authors:  Kai Zhang; Zhenqiang Fan; Yue Huang; Yuedi Ding; Minhao Xie
Journal:  Talanta       Date:  2021-09-10       Impact factor: 6.057
  2 in total

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