Alan Leviton1, Stanthia Ryan2, Elizabeth N Allred3, Raina N Fichorova2, T Michael O'Shea4, Karl Kuban5, Olaf Dammann6. 1. Neuroepidemiology Unit, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA, United States. Electronic address: alan.leviton@childrens.harvard.edu. 2. Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital Boston MA, Harvard Medical School, Boston, MA, United States. 3. Neuroepidemiology Unit, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA, United States. 4. Department of Pediatrics, University of North Carolina, Chapel Hill, NC, United States. 5. Division of Neurology, Department of Pediatrics, Boston Medical Center and Boston University, Boston, MA, United States. 6. Department of Public Health and Community Medicine, Tufts University School of Medicine, Boston, MA, United States; Perinatal Neuropidemiology Unit, Hannover Medical School, Hannover, Germany.
Abstract
BACKGROUND: To identify the antecedents and very early correlates of low concentrations of angiogenic proteins in the blood of extremely preterm newborns during the first postnatal month. METHODS: Using multiplex immunoassays we measured the concentrations of vascular endothelial growth factor A (VEGF), VEGF receptor-1 (VEGFR-1), VEGF receptor-2 (VEGFR-2), placenta growth factor (PIGF), and angiopoietins 1 and 2 (Ang-1, Ang-2), as well as 21 other proteins in blood spots collected on postnatal days 1 (N=1062), 7 (N=1087), 14 (N=989), 21 (N=940) and 28 (N=880) from infants born before the 28th week of gestation. We then sought the protein-concentration correlates of concentrations in the top and bottom quartile for gestational age and day the specimen was collected. RESULTS: Children who were delivered for medical indications and those who were severely growth restricted were more likely than others to have low day-1 blood concentrations of VEGF, VEGF-R2, Ang-1, and PIGF. Systemic inflammation accompanied top quartile concentrations of every one of the 6 angiogenic proteins. CONCLUSIONS: Low day-1 concentrations of most angiogenic proteins are associated with disorders linked to placenta insufficiency/dysfunction. High concentrations, on the other hand, are associated with systemic inflammation throughout the first postnatal month.
BACKGROUND: To identify the antecedents and very early correlates of low concentrations of angiogenic proteins in the blood of extremely preterm newborns during the first postnatal month. METHODS: Using multiplex immunoassays we measured the concentrations of vascular endothelial growth factor A (VEGF), VEGF receptor-1 (VEGFR-1), VEGF receptor-2 (VEGFR-2), placenta growth factor (PIGF), and angiopoietins 1 and 2 (Ang-1, Ang-2), as well as 21 other proteins in blood spots collected on postnatal days 1 (N=1062), 7 (N=1087), 14 (N=989), 21 (N=940) and 28 (N=880) from infants born before the 28th week of gestation. We then sought the protein-concentration correlates of concentrations in the top and bottom quartile for gestational age and day the specimen was collected. RESULTS:Children who were delivered for medical indications and those who were severely growth restricted were more likely than others to have low day-1 blood concentrations of VEGF, VEGF-R2, Ang-1, and PIGF. Systemic inflammation accompanied top quartile concentrations of every one of the 6 angiogenic proteins. CONCLUSIONS: Low day-1 concentrations of most angiogenic proteins are associated with disorders linked to placenta insufficiency/dysfunction. High concentrations, on the other hand, are associated with systemic inflammation throughout the first postnatal month.
Authors: Alan Leviton; Elizabeth N Allred; Raina N Fichorova; T Michael O'Shea; Lynn A Fordham; Karl K C Kuban; Olaf Dammann Journal: Eur J Paediatr Neurol Date: 2018-01-31 Impact factor: 3.140
Authors: Vanessa R Kay; Matthew T Rätsep; Lindsay S Cahill; Andrew F Hickman; Bruno Zavan; Margaret E Newport; Jacob Ellegood; Christine L Laliberte; James N Reynolds; Peter Carmeliet; Chandrakant Tayade; John G Sled; B Anne Croy Journal: Physiol Genomics Date: 2018-08-17 Impact factor: 3.107
Authors: Tammy Z Movsas; Kwoon Y Wong; Michael D Ober; Robert Sigler; Zhenmin M Lei; Arivalagan Muthusamy Journal: Neuroscience Date: 2018-06-08 Impact factor: 3.590
Authors: Mari Holm; Tora S Morken; Raina N Fichorova; Deborah K VanderVeen; Elizabeth N Allred; Olaf Dammann; Alan Leviton Journal: Invest Ophthalmol Vis Sci Date: 2017-12-01 Impact factor: 4.799
Authors: Steven J Korzeniewski; Elizabeth N Allred; T Michael O'Shea; Alan Leviton; Karl C K Kuban Journal: Transl Psychiatry Date: 2018-06-08 Impact factor: 6.222
Authors: Catherine M Bulka; Olaf Dammann; Hudson P Santos; Deborah K VanderVeen; Lisa Smeester; Raina Fichorova; T Michael O'Shea; Rebecca C Fry Journal: Invest Ophthalmol Vis Sci Date: 2019-07-01 Impact factor: 4.799