Literature DB >> 28502006

Fluorescence In Situ Hybridization of Cells, Chromosomes, and Formalin-Fixed Paraffin-Embedded Tissues.

Ahmad Alamri1,2, Jun Yeb Nam3, Jan K Blancato4,5.   

Abstract

Fluorescence in situ hybridization (FISH) with DNA probes allows the visualization of gene copy number and localization of specific DNA targets with fluorescence microscopy. Cells in culture, metaphase chromosomes, and tissue sections are fixed and prepared on glass slides. Both the DNA in the cells and fluorescently labeled probe are denatured, and the labeled probe is allowed to hybridize to the cellular DNA. The slides are washed, counterstained, and viewed via fluorescence microscopy. We describe the basic method for preparing slides and probes for studies involving DNA copy number changes and structural chromosome rearrangements in formalin-fixed paraffin-embedded (FFPE) tissue sections and cell culture preparations.

Entities:  

Keywords:  DNA probes; Fluorescence microscopy; Formalin-fixed paraffin-embedded tissues; Metaphase chromosomes

Mesh：

Substances：

Year:  2017        PMID： 28502006      PMCID： PMC5806521          DOI： 10.1007/978-1-4939-6990-6_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

Review 1.  Array comparative genomic hybridization and its applications in cancer.

Authors:  Daniel Pinkel; Donna G Albertson
Journal:  Nat Genet       Date:  2005-06       Impact factor: 38.330

2.  Fluorescence in situ hybridization assessment of c-myc gene amplification in breast tumor tissues.

Authors:  Jan K Blancato; Mary Steele Williams; Robert B Dickson
Journal:  Methods Mol Med       Date:  2006

3.  Detection of numerical chromosomal abnormalities by fluorescence in situ hybridization of interphase cell nuclei with chromosome-specific probes on archival cytologic samples.

Authors:  R S Cajulis; D Frias-Hidvegi; G H Yu; S Eggena
Journal:  Diagn Cytopathol       Date:  1996-03       Impact factor: 1.582

4.  Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome.

Authors:  J S Waye; H F Willard
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

Review 5.  FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue.

Authors:  Roland A Ventura; Jose I Martin-Subero; Margaret Jones; Joanna McParland; Stefan Gesk; David Y Mason; Reiner Siebert
Journal:  J Mol Diagn       Date:  2006-05       Impact factor: 5.568

6.  Cytogenetic analysis by in situ hybridization with fluorescently labeled nucleic acid probes.

Authors:  D Pinkel; J W Gray; B Trask; G van den Engh; J Fuscoe; H van Dekken
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1986

7.  Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.

Authors:  T Cremer; P Lichter; J Borden; D C Ward; L Manuelidis
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

8.  FISH-based determination of HER2 status in circulating tumor cells isolated with the microfluidic CEE™ platform.

Authors:  Julie Ann Mayer; Tam Pham; Karina L Wong; Jayne Scoggin; Edgar V Sales; Trisky Clarin; Tony J Pircher; Stephen D Mikolajczyk; Philip D Cotter; Farideh Z Bischoff
Journal:  Cancer Genet       Date:  2011-11

9.  A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.

Authors:  S-F Chin; Y Daigo; H-E Huang; N G Iyer; G Callagy; T Kranjac; M Gonzalez; T Sangan; H Earl; C Caldas
Journal:  Mol Pathol       Date:  2003-10

10.  Demecolcine effects on microtubule kinetics and on chemically assisted enucleation of bovine oocytes.

Authors:  Naiara Zoccal Saraiva; Felipe Perecin; Simone Cristina Méo; Christina Ramires Ferreira; Tatiane Almeida Drummond Tetzner; Joaquim Mansano Garcia
Journal:  Cloning Stem Cells       Date:  2009-03
View more
  5 in total

1.  Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer.

Authors:  Aftab Alam; Eric Levanduski; Parker Denz; Helena Solleiro Villavicencio; Maulasri Bhatta; Lamees Alhorebi; Yali Zhang; Eduardo Cortes Gomez; Brian Morreale; Sharon Senchanthisai; Jun Li; Steven G Turowski; Sandra Sexton; Sheila Jani Sait; Prashant K Singh; Jianmin Wang; Anirban Maitra; Pawel Kalinski; Ronald A DePinho; Huamin Wang; Wenting Liao; Scott I Abrams; Brahm H Segal; Prasenjit Dey
Journal:  Cancer Cell       Date:  2022-02-03       Impact factor: 31.743

Review 2.  The 3D Genome: From Structure to Function.

Authors:  Tapan Kumar Mohanta; Awdhesh Kumar Mishra; Ahmed Al-Harrasi
Journal:  Int J Mol Sci       Date:  2021-10-27       Impact factor: 5.923

3.  Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala.

Authors:  Maolin Lv; Xiuli Chen; Xin Huang; Ning Liu; Weimin Wang; Han Liu
Journal:  Int J Mol Sci       Date:  2021-12-01       Impact factor: 5.923

Review 4.  Discovery and Validation of Clinically Relevant Long Non-Coding RNAs in Colorectal Cancer.

Authors:  Madison Snyder; Susana Iraola-Guzmán; Ester Saus; Toni Gabaldón
Journal:  Cancers (Basel)       Date:  2022-08-10       Impact factor: 6.575

5.  Pre-Implantation Gender Selection: Family Balancing in Jordan.

Authors:  Amer Mahmoud Sindiani; Faheem Zayed; Eman Hussein Alshdaifat; Hasan M Rawashdeh; Wesam Al-Woshah; Nada Zayed
Journal:  Risk Manag Healthc Policy       Date:  2021-07-02
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.