Zhu Junbo1, Yu Yongtao2, Li Hongbo1, Zheng Fenshuang1, Lin Ruyun1, Yang Chun'ai1. 1. The Fourth Affiliated Hospital of Kunming Medical University, China. 2. School of Environmental Since and Engineering, Kunming University of Science and Technology, China. Electronic address: km_yyt@163.com.
Abstract
OBJECTIVE: This study explored the effects of sucralfate intervention as a novel treatment for paraquat (PQ) poisoning in Sprague-Dawley (SD) rats. METHODS: After PQ poisoning, the SD rats were randomly divided into the PQ control group (treated with normal saline), the sodium bicarbonate (SB) treatment group, and the sucralfate (LTL) treatment group. Then, the rats were administered normal saline, sodium bicarbonate solution, or sucralfate suspension as an intervention by gastric lavage. At 1, 3, 6, and 10days after poisoning, the left lungs of some rats were removed to determine the lung wet/dry (W/D) weight ratio. Additionally, the serum cytokine levels were measured, and the lung and kidney tissues were pathologically examined. RESULTS: After treatment, the signs and symptoms of the rats were improved, the mortality rate was reduced, the W/D weight ratio of the lung was lower, the cytokine levels [transforming growth factor (TGF)-β1, interleukin (IL)-10, and tumor necrosis factor (TNF)-α] were decreased, and the pathological injuries of the lungs and kidneys were improved. Moreover, sucralfate was significantly more effective than the control (normal saline) group and the SB treatment group. CONCLUSION: The results showed that early gastrointestinal lavage with sucralfate effectively reduced the inflammatory response and lung and kidney injuries and improved the survival of the SD rats.
OBJECTIVE: This study explored the effects of sucralfate intervention as a novel treatment for paraquat (PQ) poisoning in Sprague-Dawley (SD) rats. METHODS: After PQpoisoning, the SD rats were randomly divided into the PQ control group (treated with normal saline), the sodium bicarbonate (SB) treatment group, and the sucralfate (LTL) treatment group. Then, the rats were administered normal saline, sodium bicarbonate solution, or sucralfate suspension as an intervention by gastric lavage. At 1, 3, 6, and 10days after poisoning, the left lungs of some rats were removed to determine the lung wet/dry (W/D) weight ratio. Additionally, the serum cytokine levels were measured, and the lung and kidney tissues were pathologically examined. RESULTS: After treatment, the signs and symptoms of the rats were improved, the mortality rate was reduced, the W/D weight ratio of the lung was lower, the cytokine levels [transforming growth factor (TGF)-β1, interleukin (IL)-10, and tumor necrosis factor (TNF)-α] were decreased, and the pathological injuries of the lungs and kidneys were improved. Moreover, sucralfate was significantly more effective than the control (normal saline) group and the SB treatment group. CONCLUSION: The results showed that early gastrointestinal lavage with sucralfate effectively reduced the inflammatory response and lung and kidney injuries and improved the survival of the SD rats.
Authors: Rajeev Jaundoo; Jonathan Bohmann; Gloria E Gutierrez; Nancy Klimas; Gordon Broderick; Travis J A Craddock Journal: Mil Med Date: 2020-01-07 Impact factor: 1.437