Comprehensive Assessments of Energy Balance in Mice.

Increasing evidence supports a major role for the renin-angiotensin system (RAS) in energy balance physiology. The RAS exists as a circulating system but also as a local paracrine/autocrine signaling mechanism in target tissues including the gastrointestinal tract, the brain, the kidney, and distinct adipose beds. Through activation of various receptors in these target tissues, the RAS contributes to the control of food intake behavior, digestive efficiency, spontaneous physical activity, and aerobic and anaerobic resting metabolism. Although the assortment of methodologies available to assess the various aspects of energy balance can be daunting for an investigator new to this area, a relatively straightforward array of entry-level and advanced methodologies can be employed to comprehensively and quantitatively dissect the effects of experimental manipulations on energy homeostasis. Such methodologies and a simple initial workflow for the use of these methods are described in this chapter, including the use of metabolic caging systems, bomb calorimetry, body composition analyzers, respirometry systems, and direct calorimetry systems. Finally, a brief discussion of the statistical analyses of metabolic data is included.