| Literature DB >> 28494344 |
Guanlong Xu1, Xuxiao Zhang2, Qinfang Liu3, Guoxia Bing4, Zhe Hu2, Honglei Sun2, Xin Xiong2, Ming Jiang2, Qiming He2, Yu Wang2, Juan Pu2, Xin Guo2, Hanchun Yang2, Jinhua Liu2, Yipeng Sun5.
Abstract
Previous studies have identified a functional role of PA-X for influenza viruses in mice and avian species; however, its role in swine remains unknown. Toward this, we constructed PA-X deficient virus (Sw-FS) in the background of a Triple-reassortment (TR) H1N2 swine influenza virus (SIV) to assess the impact of PA-X in viral virulence in pigs. Expression of PA-X in TR H1N2 SIV enhanced viral replication and host protein synthesis shutoff, and inhibited the mRNA levels of type I IFNs and proinflammatory cytokines in porcine cells. A delay of proinflammatory responses was observed in lungs of pigs infected by wild type SIV (Sw-WT) compared to Sw-FS. Furthermore, Sw-WT virus replicated and transmitted more efficiently than Sw-FS in pigs. These results highlight the importance of PA-X in the moderation of virulence and immune responses of TR SIV in swine, which indicated that PA-X is a pro-virulence factor in TR SIV in pigs.Entities:
Keywords: Immune responses; PA-X protein; Swine influenza virus; Virulence
Mesh：
Substances：
Year: 2017 PMID： 28494344 DOI： 10.1016/j.virol.2017.05.002
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616