| Literature DB >> 28490391 |
X Y Wang1, X L Chen1, Z Q Huang1, D W Chen1, B Yu1, J He1, J Q Luo1, Y H Luo1, H Chen2, P Zheng1, J Yu1.
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression of target messenger RNAs (mRNAs) and miRNAs have been proven to play vital roles in skeletal muscle development. The miRNA-499-5p has been reported to be negatively related with the expression of Sox6, a critical transcription factor for the maintenance of fast-twitch skeletal muscle. In this study, we amplified a length of 2012-bp mRNA that contains a 1512-bp porcine Sox6 (pSox6) 3'UTR from skeletal muscle of a Duroc×Landrace×Yorkshire pig. By luciferase reporter assay we verified that pSox6 is a target of miR-499-5p. In extensor digitorum longus and Soleus muscles of pigs, the expression levels of miR-499-5p and pSox6 mRNA were also inversely correlated. Besides, overexpression of miR-499-5p in porcine satellite cells promoted the expression of MyHC I and MyHC IIa mRNA, along with a reduction of pSox6 mRNA. Taken together, these results indicate that miR-499-5p may facilitate the oxidative myofibers formation by downregulating pSox6 expression.Entities:
Keywords: miR-499-5p; oxidative myofibers formation; porcine Sox6zzm321990 ; porcine satellite cells; skeletal muscle
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Year: 2017 PMID： 28490391 DOI： 10.1017/S1751731117001008
Source DB: PubMed Journal: Animal ISSN： 1751-7311 Impact factor: 3.240