OBJECTIVE: We aimed at assessing the molecular adaptation of the renin-angiotensin system (RAS) after successful kidney transplantation (KTX). MATERIALS AND METHODS: In this prospective, exploratory study we analyzed 12 hemodialysis (HD) patients, who received a KTX and had excellent graft function six to 12 months thereafter. The concentrations of plasma Angiotensin (Ang) peptides (Ang I, Ang II, Ang-(1-7), Ang-(1-5), Ang-(2-8), Ang-(3-8)) were simultaneously quantified with a novel mass spectrometry-based method. Further, renin and aldosterone concentrations were determined by standard immunoassays. RESULTS: Ang values showed a strong inter-individual variability among HD patients. Yet, despite a continued broad dispersion of Ang values after KTX, a substantial improvement of the renin/Ang II correlation was observed in patients without RAS blockade or on angiotensin receptor blocker (HD: renin/Ang II R2 = 0.660, KTX: renin/Ang II R2 = 0.918). Ang-(1-7) representing the alternative RAS axis was only marginally detectable both on HD and after KTX. CONCLUSIONS: Following KTX, renin-dependent Ang II formation adapts in non-ACE inhibitor-treated patients. Thus, a largely normal RAS regulation is reconstituted after successful KTX. However, individual Ang concentration variations and a lack of potentially beneficial alternative peptides after KTX call for individualized treatment. The long-term post-transplant RAS regulation remains to be determined.
OBJECTIVE: We aimed at assessing the molecular adaptation of the renin-angiotensin system (RAS) after successful kidney transplantation (KTX). MATERIALS AND METHODS: In this prospective, exploratory study we analyzed 12 hemodialysis (HD) patients, who received a KTX and had excellent graft function six to 12 months thereafter. The concentrations of plasma Angiotensin (Ang) peptides (Ang I, Ang II, Ang-(1-7), Ang-(1-5), Ang-(2-8), Ang-(3-8)) were simultaneously quantified with a novel mass spectrometry-based method. Further, renin and aldosterone concentrations were determined by standard immunoassays. RESULTS: Ang values showed a strong inter-individual variability among HDpatients. Yet, despite a continued broad dispersion of Ang values after KTX, a substantial improvement of the renin/Ang II correlation was observed in patients without RAS blockade or on angiotensin receptor blocker (HD: renin/Ang II R2 = 0.660, KTX: renin/Ang II R2 = 0.918). Ang-(1-7) representing the alternative RAS axis was only marginally detectable both on HD and after KTX. CONCLUSIONS: Following KTX, renin-dependent Ang II formation adapts in non-ACE inhibitor-treated patients. Thus, a largely normal RAS regulation is reconstituted after successful KTX. However, individual Ang concentration variations and a lack of potentially beneficial alternative peptides after KTX call for individualized treatment. The long-term post-transplant RAS regulation remains to be determined.
Authors: Anna Gromotowicz-Poplawska; Piotr Szoka; Patrycjusz Kolodziejczyk; Karol Kramkowski; Marzena Wojewodzka-Zelezniakowicz; Ewa Chabielska Journal: Exp Biol Med (Maywood) Date: 2016-07-19
Authors: J M van Rooyen; M Poglitsch; H W Huisman; Cmc Mels; R Kruger; L Malan; S Botha; L Lammertyn; L Gafane; A E Schutte Journal: J Renin Angiotensin Aldosterone Syst Date: 2016-10-12 Impact factor: 1.636
Authors: Oleg Palygin; Denisha Spires; Vladislav Levchenko; Ruslan Bohovyk; Mykhailo Fedoriuk; Christine A Klemens; Olga Sykes; John D Bukowy; Allen W Cowley; Jozef Lazar; Daria V Ilatovskaya; Alexander Staruschenko Journal: Am J Physiol Renal Physiol Date: 2019-09-30
Authors: Johannes J Kovarik; Christopher C Kaltenecker; Chantal Kopecky; Oliver Domenig; Marlies Antlanger; Johannes Werzowa; Farsad Eskandary; Renate Kain; Marko Poglitsch; Sabine Schmaldienst; Georg A Böhmig; Marcus D Säemann Journal: Sci Rep Date: 2019-07-05 Impact factor: 4.379
Authors: Anna D Manis; Oleg Palygin; Sherif Khedr; Vladislav Levchenko; Matthew R Hodges; Alexander Staruschenko Journal: Clin Sci (Lond) Date: 2019-12-20 Impact factor: 6.124