Wesley J Moy1,2,3, Jonathan Yao4, Sébastien M de Feraudy4, Sean M White1, Jocelynda Salvador1,2, Kristen M Kelly1,4,5, Bernard Choi1,2,5,6. 1. Beckman Laser Institute and Medical Clinic, University of California, Irvine, California. 2. Department of Biomedical Engineering, University of California, Irvine, California. 3. Department of Otolaryngology, University of California, Irvine, California. 4. Department of Dermatology, University of California, Irvine, California. 5. Department of Surgery, University of California, Irvine, California. 6. Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, California.
Abstract
BACKGROUND AND OBJECTIVE: Alternative treatments are needed to achieve consistent and more complete port wine stain (PWS) removal, especially in darker skin types; photodynamic therapy (PDT) is a promising alternative treatment. To this end, we previously reported on Talaporfin Sodium (TS)-mediated PDT. It is essential to understand treatment tissue effects to design a protocol that will achieve selective vascular injury without ulceration and scarring. The objective of this work is to assess skin changes associated with TS-mediated PDT with clinically relevant treatment parameters. STUDY DESIGN/ MATERIALS AND METHODS: We performed TS (0.75 mg/kg)-mediated PDT (664 nm) on Sprague Dawley rats. Radiant exposures were varied between 15 and 100 J/cm2 . We took skin biopsies from subjects at 9 hours following PDT. We assessed the degree and depth of vascular and surrounding tissue injury using histology and immunohistochemical staining. RESULTS: TS-mediated PDT at 0.75 mg/kg combined with 15 and 25 J/cm2 light doses resulted in vascular injury with minimal epidermal damage. At light dose of 50 J/cm2 , epidermal damage was noted with vascular injury. At light doses >50 J/cm2 , both vascular and surrounding tissue injury were observed in the forms of vasculitis, extravasated red blood cells, and coagulative necrosis. Extensive coagulative necrosis involving deeper adnexal structures was observed for 75 and 100 J/cm2 light doses. Observed depth of injury increased with increasing radiant exposure, although this relationship was not linear. CONCLUSION: TS-mediated PDT can cause selective vascular injury; however, at higher light doses, significant extra-vascular injury was observed. This information can be used to contribute to design of safe protocols to be used for treatment of cutaneous vascular lesions. Lasers Surg. Med. 49:767-772, 2017.
BACKGROUND AND OBJECTIVE: Alternative treatments are needed to achieve consistent and more complete port wine stain (PWS) removal, especially in darker skin types; photodynamic therapy (PDT) is a promising alternative treatment. To this end, we previously reported on Talaporfin Sodium (TS)-mediated PDT. It is essential to understand treatment tissue effects to design a protocol that will achieve selective vascular injury without ulceration and scarring. The objective of this work is to assess skin changes associated with TS-mediated PDT with clinically relevant treatment parameters. STUDY DESIGN/ MATERIALS AND METHODS: We performed TS (0.75 mg/kg)-mediated PDT (664 nm) on Sprague Dawley rats. Radiant exposures were varied between 15 and 100 J/cm2 . We took skin biopsies from subjects at 9 hours following PDT. We assessed the degree and depth of vascular and surrounding tissue injury using histology and immunohistochemical staining. RESULTS:TS-mediated PDT at 0.75 mg/kg combined with 15 and 25 J/cm2 light doses resulted in vascular injury with minimal epidermal damage. At light dose of 50 J/cm2 , epidermal damage was noted with vascular injury. At light doses >50 J/cm2 , both vascular and surrounding tissue injury were observed in the forms of vasculitis, extravasated red blood cells, and coagulative necrosis. Extensive coagulative necrosis involving deeper adnexal structures was observed for 75 and 100 J/cm2 light doses. Observed depth of injury increased with increasing radiant exposure, although this relationship was not linear. CONCLUSION:TS-mediated PDT can cause selective vascular injury; however, at higher light doses, significant extra-vascular injury was observed. This information can be used to contribute to design of safe protocols to be used for treatment of cutaneous vascular lesions. Lasers Surg. Med. 49:767-772, 2017.
Authors: Bernard Choi; Wenbin Tan; Wangcun Jia; Sean M White; Wesley J Moy; Bruce Y Yang; Jiang Zhu; Zhongping Chen; Kristen M Kelly; J Stuart Nelson Journal: IEEE J Sel Top Quantum Electron Date: 2015-10-26 Impact factor: 4.544
Authors: Wesley J Moy; Shreyas J Patel; Ben S Lertsakdadet; Rajan P Arora; Katherine M Nielsen; Kristen M Kelly; Bernard Choi Journal: Lasers Surg Med Date: 2012-02 Impact factor: 4.025
Authors: Guillermo Aguilar; Bernard Choi; Mans Broekgaarden; Owen Yang; Bruce Yang; Pedram Ghasri; Jennifer K Chen; Rick Bezemer; J Stuart Nelson; Anne Margreet van Drooge; Albert Wolkerstorfer; Kristen M Kelly; Michal Heger Journal: Ann Biomed Eng Date: 2011-10-21 Impact factor: 3.934