Literature DB >> 2848841

Activation mechanism of calcium-activated neutral protease. Evidence for the existence of intramolecular and intermolecular autolyses.

M Inomata1, Y Kasai, M Nakamura, S Kawashima.   

Abstract

The activation mechanism through limited autolysis of a calcium-activated neutral protease (CANP) with a high sensitivity to calcium ions (microCANP) was analyzed. The rate of autolysis was dependent on microCANP concentration. The reaction was inhibited by high concentrations of digestible substrates but not by a nondigestible substrate. Incubation of microCANP inactivated by N-ethylmaleimide with a small amount of activated microCANP caused the degradation of the former in a manner similar to the autolysis of native microCANP. Immobilized microCANP bound to an anti-microCANP immunoglobulin G column autolyzed on addition of calcium ions. These results show that activation of microCANP through limited autolysis involves both intramolecular and intermolecular reactions.

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Year:  1988        PMID： 2848841

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  8 in total

1.  Activation of calpain I in thrombin-stimulated platelets is regulated by the initial elevation of the cytosolic Ca2+ concentration.

Authors:  H Ishii; Y Suzuki; M Kuboki; M Morikawa; M Inoue; M Kazama
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

2.  Collagenase-assisted wound bed preparation: An in vitro comparison between Vibrio alginolyticus and Clostridium histolyticum collagenases on substrate specificity.

Authors:  Roberta Di Pasquale; Susanna Vaccaro; Michele Caputo; Christian Cuppari; Salvatore Caruso; Angela Catania; Luciano Messina
Journal:  Int Wound J       Date:  2019-05-31       Impact factor: 3.315

3.  Calpain (Ca(2+)-dependent thiol protease) in erythrocytes of young and old individuals.

Authors:  T Glaser; N Schwarz-Benmeir; S Barnoy; S Barak; Z Eshhar; N S Kosower
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-16       Impact factor: 11.205

4.  N-ethylmaleimide activates a Cl(-)-independent component of K(+) flux in mouse erythrocytes.

Authors:  Boris E Shmukler; Ann Hsu; Jessica Alves; Marie Trudel; Marco B Rust; Christian A Hubner; Alicia Rivera; Seth L Alper
Journal:  Blood Cells Mol Dis       Date:  2013-03-06       Impact factor: 3.039

Review 5.  The pathogenic activation of calpain: a marker and mediator of cellular toxicity and disease states.

Authors:  P W Vanderklish; B A Bahr
Journal:  Int J Exp Pathol       Date:  2000-10       Impact factor: 1.925

6.  Autolysis parallels activation of mu-calpain.

Authors:  A Baki; P Tompa; A Alexa; O Molnár; P Friedrich
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

Review 7.  Calpain chronicle--an enzyme family under multidisciplinary characterization.

Authors:  Hiroyuki Sorimachi; Shoji Hata; Yasuko Ono
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2011       Impact factor: 3.493

8.  Host Cell Calpains Can Cleave Structural Proteins from the Enterovirus Polyprotein.

Authors:  Mira Laajala; Minna M Hankaniemi; Juha A E Määttä; Vesa P Hytönen; Olli H Laitinen; Varpu Marjomäki
Journal:  Viruses       Date:  2019-11-28       Impact factor: 5.048
  8 in total

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