Literature DB >> 2848717

The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line.

H Doostdar1, S J Duthie, M D Burke, W T Melvin, M H Grant.   

Abstract

When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.

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Year:  1988        PMID： 2848717     DOI： 10.1016/0014-5793(88)81021-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

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2.  Co-variation of glutathione transferase expression and cytostatic drug resistance in HeLa cells: establishment of class Mu glutathione transferase M3-3 as the dominating isoenzyme.

Authors:  X Y Hao; M Widersten; M Ridderström; U Hellman; B Mannervik
Journal:  Biochem J       Date:  1994-01-01       Impact factor: 3.857

Review 3.  Preclinical models of idiosyncratic drug-induced liver injury (iDILI): Moving towards prediction.

Authors:  Antonio Segovia-Zafra; Daniel E Di Zeo-Sánchez; Carlos López-Gómez; Zeus Pérez-Valdés; Eduardo García-Fuentes; Raúl J Andrade; M Isabel Lucena; Marina Villanueva-Paz
Journal:  Acta Pharm Sin B       Date:  2021-11-18       Impact factor: 11.413

4.  Characterization of the human hepatocellular carcinoma (hepg2) cell line as an in vitro model for cadmium toxicity studies.

Authors:  P F Dehn; C M White; D E Conners; G Shipkey; T A Cumbo
Journal:  In Vitro Cell Dev Biol Anim       Date:  2004 May-Jun       Impact factor: 2.416

5.  Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver.

Authors:  S L Nyberg; R P Remmel; H J Mann; M V Peshwa; W S Hu; F B Cerra
Journal:  Ann Surg       Date:  1994-07       Impact factor: 12.969

6.  Evaluation of the potent anti-hepatitis B virus agent (-) cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine in a novel in vivo model.

Authors:  L D Condreay; R W Jansen; T F Powdrill; L C Johnson; D W Selleseth; M T Paff; S M Daluge; G R Painter; P A Furman; M N Ellis
Journal:  Antimicrob Agents Chemother       Date:  1994-03       Impact factor: 5.191

7.  Modulation by extracellular matrices of monooxygenase and CYP1A1 induction in Hep G2 cells in serum-free culture.

Authors:  A Nakama
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

8.  Similarities and differences in the expression of drug-metabolizing enzymes between human hepatic cell lines and primary human hepatocytes.

Authors:  Lei Guo; Stacey Dial; Leming Shi; William Branham; Jie Liu; Jia-Long Fang; Bridgett Green; Helen Deng; Jim Kaput; Baitang Ning
Journal:  Drug Metab Dispos       Date:  2010-12-13       Impact factor: 3.922
  8 in total

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