| Literature DB >> 28484016 |
Alexander H Tuttle1,2, Shannon Tansley1,2, Kimberly Dossett1,2, Sarasa Tohyama1,2, Arkady Khoutorsky3,4, Sioui Maldonado-Bouchard1,2, Liane Stein1,2, Lindsey Gerstein1,2, Hayley Crawhall-Duk1,2, Rebecca Pearl1,2, Melissa Sukosd1,2, Philip Leger1,2, Oliver M Hardt5,6, David Yachnin1,2, Jean-Sebastien Austin1,2, Claire M Chan7, Tine Pooters6,8, Isabelle Groves6,8, Loren J Martin7, Nahum Sonenberg9,4, Christos G Gkogkas10,8, Jeffrey S Mogil11,2.
Abstract
Existing assays of social interaction are suboptimal, and none measures propinquity, the tendency of rodents to maintain close physical proximity. These assays are ubiquitously performed using inbred mouse strains and mutations placed on inbred genetic backgrounds. We developed the automatable tube cooccupancy test (TCOT) based on propinquity, the tendency of freely mobile rodents to maintain close physical proximity, and assessed TCOT behavior on a variety of genotypes and social and environmental conditions. In outbred mice and rats, familiarity determined willingness to cooccupy the tube, with siblings and/or cagemates of both sexes exhibiting higher cooccupancy behavior than strangers. Subsequent testing using multiple genotypes revealed that inbred strain siblings do not cooccupy at higher rates than strangers, in marked contrast to both outbred and rederived wild mice. Mutant mouse strains with "autistic-like" phenotypes (Fmr1-/y and Eif4e Ser209Ala) displayed significantly decreased cooccupancy.Entities:
Keywords: autism; genetics; propinquity; rodent behavior; social interaction
Mesh：
Year: 2017 PMID： 28484016 PMCID： PMC5448193 DOI： 10.1073/pnas.1703477114
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205