Jennifer Bolton1,2, Emilie Montastier1,2,3, Jérôme Carayol4, Sophie Bonnel1,2, Lucile Mir1,2, Marie-Adeline Marques1,2, Arne Astrup5, Wim Saris6, Jason Iacovoni1,2, Nathalie Villa-Vialaneix7, Armand Valsesia4, Dominique Langin1,2,3, Nathalie Viguerie1,2. 1. Institut National de la Santé et de la Recherche Médicale, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France. 2. University of Toulouse, Paul Sabatier University, 31400 Toulouse, France. 3. Toulouse University Hospitals, Departments of Endocrinology, Metabolism and Nutrition, 31400 Toulouse, France. 4. Nestlé Institute of Health Sciences SA, CH-1015 Lausanne, Switzerland. 5. Department of Nutrition, Exercise and Sports, Faculty of Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. 6. Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, 6229 Maastricht, The Netherlands. 7. Unité de Mathématiques et Informatique Appliquées de Toulouse, Université de Toulouse, 31326 Castanet Tolosan, France.
Abstract
Context: Although calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals. Objective: To identify biomarkers of successful weight control during a dietary intervention (DI). Design, Setting, and Participants: Adipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay. Intervention: Individuals underwent 8 weeks of low-calorie diet, then 6 months of ad libitum diet. Outcome Measure: Weight changes at the end of the DI. Results: We evaluated six genes that had altered expression during DI, encode secreted proteins, and have not previously been implicated in weight control (EGFL6, FSTL3, CRYAB, TNMD, SPARC, IGFBP3), as well as genes for which baseline expression differed between those with good and poor weight control (ASPN, USP53). Changes in plasma concentrations of EGFL6, FSTL3, and CRYAB mirrored AT messenger RNA expression; all decreased during DI in individuals with good weight control. ASPN and USP53 had higher baseline expression in individuals who went on to have good weight control. Expression quantitative trait loci analysis found polymorphisms associated with expression levels of USP53 in AT. A regulatory network was identified in which transforming growth factor β1 (TGF-β1) was responsible for downregulation of certain genes during DI in good controllers. Interestingly, ASPN is a TGF-β1 inhibitor. Conclusions: We found circulating biomarkers associated with weight control that could influence weight management strategies and genes that may be prognostic for successful weight control.
Context: Although calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals. Objective: To identify biomarkers of successful weight control during a dietary intervention (DI). Design, Setting, and Participants: Adipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay. Intervention: Individuals underwent 8 weeks of low-calorie diet, then 6 months of ad libitum diet. Outcome Measure: Weight changes at the end of the DI. Results: We evaluated six genes that had altered expression during DI, encode secreted proteins, and have not previously been implicated in weight control (EGFL6, FSTL3, CRYAB, TNMD, SPARC, IGFBP3), as well as genes for which baseline expression differed between those with good and poor weight control (ASPN, USP53). Changes in plasma concentrations of EGFL6, FSTL3, and CRYAB mirrored AT messenger RNA expression; all decreased during DI in individuals with good weight control. ASPN and USP53 had higher baseline expression in individuals who went on to have good weight control. Expression quantitative trait loci analysis found polymorphisms associated with expression levels of USP53 in AT. A regulatory network was identified in which transforming growth factor β1 (TGF-β1) was responsible for downregulation of certain genes during DI in good controllers. Interestingly, ASPN is a TGF-β1 inhibitor. Conclusions: We found circulating biomarkers associated with weight control that could influence weight management strategies and genes that may be prognostic for successful weight control.
Authors: Qi Qiao; Freek G Bouwman; Marleen A van Baak; Nadia J T Roumans; Roel G Vink; Susan L M Coort; Johan W Renes; Edwin C M Mariman Journal: Adipocyte Date: 2019-12 Impact factor: 4.534
Authors: David R Powell; Jean-Pierre Revelli; Deon D Doree; Christopher M DaCosta; Urvi Desai; Melanie K Shadoan; Lawrence Rodriguez; Michael Mullens; Qi M Yang; Zhi-Ming Ding; Laura L Kirkpatrick; Peter Vogel; Brian Zambrowicz; Arthur T Sands; Kenneth A Platt; Gwenn M Hansen; Robert Brommage Journal: Diabetes Metab Syndr Obes Date: 2021-08-28 Impact factor: 3.168
Authors: Augusto Anguita-Ruiz; Mireia Bustos-Aibar; Julio Plaza-Díaz; Andrea Mendez-Gutierrez; Jesús Alcalá-Fdez; Concepción María Aguilera; Francisco Javier Ruiz-Ojeda Journal: Int J Mol Sci Date: 2021-03-09 Impact factor: 5.923