Literature DB >> 2848180

Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens.

S Urasawa1, T Urasawa, K Taniguchi, Y Morita, N Sakurada, Y Saeki, O Morita, S Hasegawa.   

Abstract

We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antisera (NT serotyping) in the sensitivity and specificity of the test. Direct serotyping of HRVs which were contained in stool specimens indicated that while only 37% of the samples were successfully serotyped in NT, 78% of the samples could be serotyped in ELISA. Regarding the samples whose serotype could be determined in the two tests, the assigned serotypes were identical in both tests. The results obtained indicated the utility of ELISA serotyping in clinical and epidemiologic studies of HRV infection.

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Year:  1988        PMID： 2848180     DOI： 10.1111/j.1348-0421.1988.tb01431.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  15 in total

1.  Operational overlapping of cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus serotype 3.

Authors:  N Kobayashi; K Taniguchi; S Urasawa
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

2.  Epidemiology of rotavirus serotypes in Melbourne, Australia, from 1973 to 1989.

Authors:  R F Bishop; L E Unicomb; G L Barnes
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

3.  Preparation and characterization of a neutralizing monoclonal antibody directed to VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes.

Authors:  N Kobayashi; K Taniguchi; T Urasawa; S Urasawa
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

4.  Characterization by enzyme-linked immunosorbent assay using subgroup- and serotype-specific monoclonal antibodies of human rotavirus obtained from diarrheic patients in Bangladesh.

Authors:  M U Ahmed; K Taniguchi; N Kobayashi; T Urasawa; F Wakasugi; M Islam; H Shaikh; S Urasawa
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

5.  Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype.

Authors:  N Kobayashi; I C Lintag; T Urasawa; K Taniguchi; M C Saniel; S Urasawa
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

Review 6.  Rotavirus gene structure and function.

Authors:  M K Estes; J Cohen
Journal:  Microbiol Rev       Date:  1989-12

7.  Effect of the selection pressure with anti-VP7 and anti-VP4 neutralizing monoclonal antibodies on reassortant formation between two human rotaviruses.

Authors:  N Kobayashi; K Taniguchi; T Urasawa; S Urasawa
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

8.  Subgroup and serotype distributions of human, bovine, and porcine rotavirus in Thailand.

Authors:  Y Pongsuwanne; K Taniguchi; M Choonthanom; M Chiwakul; T Susansook; S Saguanwongse; C Jayavasu; S Urasawa
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

9.  Serological characterization of bovine rotaviruses isolated from dairy and beef herds in Argentina.

Authors:  R C Bellinzoni; J O Blackhall; N M Mattion; M K Estes; D R Snodgrass; J L LaTorre; E A Scodeller
Journal:  J Clin Microbiol       Date:  1989-11       Impact factor: 5.948

10.  Analysis of human rotavirus strains prevailing in Bangladesh in relation to nationwide floods brought by the 1988 monsoon.

Authors:  M U Ahmed; S Urasawa; K Taniguchi; T Urasawa; N Kobayashi; F Wakasugi; A I Islam; H A Sahikh
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948
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