Literature DB >> 28481728

A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system.

Li Zhang1, Qianqian Li1, Qiang Liu1, Weijin Huang1, Jianhui Nie1, Youchun Wang1.   

Abstract

Marburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In this study, a high titer of MARV pseudovirus was generated through optimization of the HIV backbone vectors, the ratio of backbone vector to MARV glycoprotein expression vector, and the transfection reagents. An in vitro neutralization assay and an in vivo bioluminescent imaging mouse model for MARV were developed based on the pseudovirus. Protective serum against MARV was successfully induced in guinea pigs, which showed high neutralization activity in vitro and could also protect Balb/c mice from MARV pseudovirus infection in vivo. This system could be a convenient tool to enable the evaluation of vaccines and therapeutic drugs against MARV in non-BSL-4 laboratories.

Entities:  

Keywords:  animal model; bioluminescent imaging; marburg virus; passive protection; pseudovirus

Mesh：

Substances：

Year:  2017        PMID： 28481728      PMCID： PMC5557217          DOI： 10.1080/21645515.2017.1325050

Source DB:  PubMed          Journal:  Hum Vaccin Immunother        ISSN： 2164-5515            Impact factor:   3.452


  26 in total

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4.  Noninvasive bioluminescence imaging in small animals.

Authors:  Kurt R Zinn; Tandra R Chaudhuri; April Adams Szafran; Darrell O'Quinn; Casey Weaver; Kari Dugger; Dale Lamar; Robert A Kesterson; Xiangdong Wang; Stuart J Frank
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5.  Discovery, synthesis, and biological evaluation of a novel group of selective inhibitors of filoviral entry.

Authors:  Maria V Yermolina; Jizhen Wang; Michael Caffrey; Lijun L Rong; Duncan J Wardrop
Journal:  J Med Chem       Date:  2011-01-04       Impact factor: 7.446

6.  Tyro3 family-mediated cell entry of Ebola and Marburg viruses.

Authors:  Masayuki Shimojima; Ayato Takada; Hideki Ebihara; Gabriele Neumann; Kouki Fujioka; Tatsuro Irimura; Steven Jones; Heinz Feldmann; Yoshihiro Kawaoka
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Authors:  Daniel A Ozaki; Hongmei Gao; Christopher A Todd; Kelli M Greene; David C Montefiori; Marcella Sarzotti-Kelsoe
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Review 10.  Forty-five years of Marburg virus research.

Authors:  Kristina Brauburger; Adam J Hume; Elke Mühlberger; Judith Olejnik
Journal:  Viruses       Date:  2012-10-01       Impact factor: 5.048
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4.  Development of in vitro and in vivo neutralization assays based on the pseudotyped H7N9 virus.

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6.  Synthesis and biological evaluation of novel tricyclic matrinic derivatives as potential anti-filovirus agents.

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7.  An optimized and robust SARS-CoV-2 pseudovirus system for viral entry research.

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8.  Establishment of pseudovirus infection mouse models for in vivo pharmacodynamics evaluation of filovirus entry inhibitors.

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Review 9.  Current status on the development of pseudoviruses for enveloped viruses.

Authors:  Qianqian Li; Qiang Liu; Weijin Huang; Xuguang Li; Youchun Wang
Journal:  Rev Med Virol       Date:  2017-12-07       Impact factor: 6.989

10.  Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2.

Authors:  Jianhui Nie; Qianqian Li; Jiajing Wu; Chenyan Zhao; Huan Hao; Huan Liu; Li Zhang; Lingling Nie; Haiyang Qin; Meng Wang; Qiong Lu; Xiaoyu Li; Qiyu Sun; Junkai Liu; Changfa Fan; Weijin Huang; Miao Xu; Youchun Wang
Journal:  Emerg Microbes Infect       Date:  2020-12       Impact factor: 7.163
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