Kyung-Jin Park1, Rainer Haak2, Dirk Ziebolz3, Felix Krause4, Hartmut Schneider5. 1. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103 Leipzig, Germany. Electronic address: kyungjin.park@medizin.uni-leipzig.de. 2. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103 Leipzig, Germany. Electronic address: rainer.haak@medizin.uni-leipzig.de. 3. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103 Leipzig, Germany. Electronic address: dirk.ziebolz@medizin.uni-leipzig.de. 4. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103 Leipzig, Germany. Electronic address: felix.krause@medizin.uni-leipzig.de. 5. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Liebig Str. 12, Haus 1, 04103 Leipzig, Germany. Electronic address: hartmut.schneider@medizin.uni-leipzig.de.
Abstract
OBJECTIVES: This study evaluated (1) the detection and assessment of non-cavitated occlusal carious lesions by spectral domain optical coherence tomography (SD-OCT) and (2) the impact of varying angle of incidence (AI) of probe light and refractive index matching (RIM). METHODS: Nine extracted human molars with 18 occlusal lesions (ICDAS code 2) were visually selected. 18 regions of interest (ROI) were imaged with SD-OCT under varying AI (0°, ±5°, ±10°, ±15°) and with/without application of glycerine at 0°. X-ray micro computed tomography (μCT) was used as a validation standard. μCT and OCT signals were categorized according to the lesion extent: 1-sound, 2-lesion limited to half of enamel, 3-lesion limited to enamel, 4-lesion into dentin. Agreement between both methods was assessed. Intra- and inter-examiner reproducibility analyses were conducted. STATISTICS: Cohen's kappa coefficient (κ), Spearman's rho correlation (rs) and Wilcoxon test (α=0.05). RESULTS: Slight to moderate agreement (κ=0.153) between μCT and OCT was obtained at an AI of 0° (Wilcoxon: p=0.02). With variation of Al a substantial agreement (κ=0.607) was observed (p=0.74). Spearman's correlation between both methods was 0.428 at 0°, 0.75 with varying AI and 0.573 with glycerine. Kappa values for intra-and inter-examiner analysis ranged between 0.81 and 0.88 and between 0.25 and 0.73, respectively. CONCLUSION: Variation of AI improves the detectability of non-cavitated occlusal carious lesions. RIM can enhance signal-to-noise ratio. CLINICAL RELEVANCE: OCT could provide additional diagnostic information in single and longitudinal assessments of occlusal carious lesions.
OBJECTIVES: This study evaluated (1) the detection and assessment of non-cavitated occlusal carious lesions by spectral domain optical coherence tomography (SD-OCT) and (2) the impact of varying angle of incidence (AI) of probe light and refractive index matching (RIM). METHODS: Nine extracted human molars with 18 occlusal lesions (ICDAS code 2) were visually selected. 18 regions of interest (ROI) were imaged with SD-OCT under varying AI (0°, ±5°, ±10°, ±15°) and with/without application of glycerine at 0°. X-ray micro computed tomography (μCT) was used as a validation standard. μCT and OCT signals were categorized according to the lesion extent: 1-sound, 2-lesion limited to half of enamel, 3-lesion limited to enamel, 4-lesion into dentin. Agreement between both methods was assessed. Intra- and inter-examiner reproducibility analyses were conducted. STATISTICS: Cohen's kappa coefficient (κ), Spearman's rho correlation (rs) and Wilcoxon test (α=0.05). RESULTS: Slight to moderate agreement (κ=0.153) between μCT and OCT was obtained at an AI of 0° (Wilcoxon: p=0.02). With variation of Al a substantial agreement (κ=0.607) was observed (p=0.74). Spearman's correlation between both methods was 0.428 at 0°, 0.75 with varying AI and 0.573 with glycerine. Kappa values for intra-and inter-examiner analysis ranged between 0.81 and 0.88 and between 0.25 and 0.73, respectively. CONCLUSION: Variation of AI improves the detectability of non-cavitated occlusal carious lesions. RIM can enhance signal-to-noise ratio. CLINICAL RELEVANCE: OCT could provide additional diagnostic information in single and longitudinal assessments of occlusal carious lesions.
Authors: Sinan Şen; Ralf Erber; Kevin Kunzmann; Stefanie Kirschner; Vanessa Weyer; Lothar Schilling; Marc A Brockmann; Stefan Rues; Gül Orhan; Christopher J Lux; Sebastian Zingler Journal: Clin Oral Investig Date: 2018-03-09 Impact factor: 3.573
Authors: Charlotte Theresa Trebing; Sinan Sen; Stefan Rues; Christopher Herpel; Maria Schöllhorn; Christopher J Lux; Peter Rammelsberg; Franz Sebastian Schwindling Journal: Heliyon Date: 2021-04-08