Literature DB >> 28478554

A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis.

Patrick Cherubin1, Beatriz Quiñones2, Salem Elkahoui3, Wallace Yokoyama4, Ken Teter5.   

Abstract

Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.

Keywords:  AB toxin; Ricin; Shiga toxin; Toxicity assay; Toxin detection; Toxin inhibitors; Vero cells

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Substances：

Year:  2017        PMID： 28478554     DOI： 10.1007/978-1-4939-6958-6_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Bimodal Response to Shiga Toxin 2 Subtypes Results from Relatively Weak Binding to the Target Cell.

Authors:  Patrick Cherubin; Dennis Fidler; Beatriz Quiñones; Ken Teter
Journal:  Infect Immun       Date:  2019-11-18       Impact factor: 3.441

2.  Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy.

Authors:  Efi Makdasi; Orly Laskar; Elad Milrot; Ofir Schuster; Shlomo Shmaya; Shmuel Yitzhaki
Journal:  Toxins (Basel)       Date:  2019-03-22       Impact factor: 4.546

3.  Top-Down Proteomic Identification of Shiga Toxin 1 and 2 from Pathogenic Escherichia coli Using MALDI-TOF-TOF Tandem Mass Spectrometry.

Authors:  Clifton K Fagerquist; William J Zaragoza; Michelle Q Carter
Journal:  Microorganisms       Date:  2019-10-25

4.  Cellular recovery from exposure to sub-optimal concentrations of AB toxins that inhibit protein synthesis.

Authors:  Patrick Cherubin; Beatriz Quiñones; Ken Teter
Journal:  Sci Rep       Date:  2018-02-06       Impact factor: 4.379
  4 in total

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