Katja Dressen1, Natalie Hermann1, Steffen Manekeller2,3, Gisela Walgenbach-Bruenagel2,3, Frank A Schildberg4, Karina Hettwer5,6, Steffen Uhlig5,6, Jörg C Kalff2,3, Gunther Hartmann1,3, Stefan Holdenrieder7,3,6. 1. Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, Germany. 2. Department of Surgery, University Hospital Bonn, Bonn, Germany. 3. Center for Integrated Oncology (CIO), Köln/Bonn, Bonn, Germany. 4. Institutes of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany. 5. QuoData Statistics GmbH, Dresden, Germany. 6. Joint Research and Services Center for Biomarker Evaluation in Oncology, Bonn/Dresden, Germany. 7. Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, Germany Stefan.Holdenrieder@uni-bonn.de.
Abstract
BACKGROUND/AIM: We evaluated the diagnostic performance of a newly-launched magnetic bead-based multiplex immunoassay panel including cancer, apoptotic, immunological and angiogenesis biomarkers for differential diagnosis of colorectal cancer (CRC). PATIENTS AND METHODS: Serum samples of 106 individuals comprising of 35 patients with CRC (23 colon cancer, 12 rectal cancer), 20 with respective benign colorectal diseases and 51 healthy controls were analyzed by the Milliplex™ MAP Human Circulating Cancer Biomarker Panel 1 run on the Bio-Plex™ 200 System. RESULTS: IL-8, CEA, HGF, TNFα, CYFRA 21-1, OPN, TGFα, CA 19-9, CA 125, AFP and sFas showed significantly higher levels in cancer samples compared to healthy controls. It is noteworthy that comparing CRC and benign colorectal disease samples, many immunological and cell death markers were elevated as well. Exclusively, six markers were distinguished significantly between both groups: CEA showed the best performance in differential diagnosis reaching an AUC of 0.859 in ROC curve followed by CA 19-9, CYFRA 21-1, IL-8, CA 125 and OPN reaching AUCs between 0.696 and 0.744. Correlation with tumor stage was found for CEA, sFas and CYFRA 21-1. Finally marker scores were assembled showing that a combination of CEA and CA 19-9 had a higher AUC (0.893) compared to the biomarkers alone. CONCLUSION: Differential diagnosis of CRC can be improved by new biomarker classes and their combination assessed by novel multiplex immunoassay. Copyright
BACKGROUND/AIM: We evaluated the diagnostic performance of a newly-launched magnetic bead-based multiplex immunoassay panel including cancer, apoptotic, immunological and angiogenesis biomarkers for differential diagnosis of colorectal cancer (CRC). PATIENTS AND METHODS: Serum samples of 106 individuals comprising of 35 patients with CRC (23 colon cancer, 12 rectal cancer), 20 with respective benign colorectal diseases and 51 healthy controls were analyzed by the Milliplex™ MAP Human Circulating Cancer Biomarker Panel 1 run on the Bio-Plex™ 200 System. RESULTS:IL-8, CEA, HGF, TNFα, CYFRA 21-1, OPN, TGFα, CA 19-9, CA 125, AFP and sFas showed significantly higher levels in cancer samples compared to healthy controls. It is noteworthy that comparing CRC and benign colorectal disease samples, many immunological and cell death markers were elevated as well. Exclusively, six markers were distinguished significantly between both groups: CEA showed the best performance in differential diagnosis reaching an AUC of 0.859 in ROC curve followed by CA 19-9, CYFRA 21-1, IL-8, CA 125 and OPN reaching AUCs between 0.696 and 0.744. Correlation with tumor stage was found for CEA, sFas and CYFRA 21-1. Finally marker scores were assembled showing that a combination of CEA and CA 19-9 had a higher AUC (0.893) compared to the biomarkers alone. CONCLUSION: Differential diagnosis of CRC can be improved by new biomarker classes and their combination assessed by novel multiplex immunoassay. Copyright
Authors: Siwen Hu-Lieskovan; Srabani Bhaumik; Kavita Dhodapkar; Jean-Charles J B Grivel; Sumati Gupta; Brent A Hanks; Sylvia Janetzki; Thomas O Kleen; Yoshinobu Koguchi; Amanda W Lund; Cristina Maccalli; Yolanda D Mahnke; Ruslan D Novosiadly; Senthamil R Selvan; Tasha Sims; Yingdong Zhao; Holden T Maecker Journal: J Immunother Cancer Date: 2020-12 Impact factor: 13.751