BACKGROUND/AIM: SLC9A9 plays an oncogenic role in esophageal squamous carcinoma and glioblastoma. Herein, we showed an oncogenic function of SLC9A9 in colorectal cancer (CRC). MATERIALS AND METHODS: We examined SLC9A9 expression in CRC specimens by immunohistochemistry. In CRC tissues, the relationship between SLC9A9 expression and clinicopathological factors was further elucidated by quantitative real-time polymerase chain reaction (qRT-PCR) and gene set enrichment analysis (GSEA). In vitro, we performed knockdown and overexpression experiments. RESULTS: SLC9A9 was overexpressed in CRC specimens. In clinicopathological analysis of our cohort, high SLC9A9 expression increased liver metastasis and was correlated with worse prognoses in two cohorts. A significantly positive relationship between SLC9A9 and EGFR was revealed. While knockdown of SLC9A9 suppressed proliferation and anchorage-independent growth, up-regulation of SLC9A9 promoted proliferation and anchorage-independent growth in vitro. CONCLUSION: SLC9A9 has an oncogenic function by being related to EGFR signaling, suggesting SLC9A9 may be a novel prognostic indicator and a therapeutic target in CRC. Copyright
BACKGROUND/AIM: SLC9A9 plays an oncogenic role in esophageal squamous carcinoma and glioblastoma. Herein, we showed an oncogenic function of SLC9A9 in colorectal cancer (CRC). MATERIALS AND METHODS: We examined SLC9A9 expression in CRC specimens by immunohistochemistry. In CRC tissues, the relationship between SLC9A9 expression and clinicopathological factors was further elucidated by quantitative real-time polymerase chain reaction (qRT-PCR) and gene set enrichment analysis (GSEA). In vitro, we performed knockdown and overexpression experiments. RESULTS:SLC9A9 was overexpressed in CRC specimens. In clinicopathological analysis of our cohort, high SLC9A9 expression increased liver metastasis and was correlated with worse prognoses in two cohorts. A significantly positive relationship between SLC9A9 and EGFR was revealed. While knockdown of SLC9A9 suppressed proliferation and anchorage-independent growth, up-regulation of SLC9A9 promoted proliferation and anchorage-independent growth in vitro. CONCLUSION:SLC9A9 has an oncogenic function by being related to EGFR signaling, suggesting SLC9A9 may be a novel prognostic indicator and a therapeutic target in CRC. Copyright
Authors: Maximilian M L Knott; Thomas G P Grünewald; Florencia Cidre-Aranaz; Jing Li; Tilman L B Hölting; Martin F Orth; Roland Imle; Stefanie Kutschmann; Giulia Ammirati; Katharina Ceranski; Martha Julia Carreño-Gonzalez; Merve Kasan; Aruna Marchetto; Cornelius M Funk; Felix Bestvater; Simone Bersini; Chiara Arrigoni; Matteo Moretti; Uwe Thiel; Daniel Baumhoer; Felix Sahm; Stefan M Pfister; Wolfgang Hartmann; Uta Dirksen; Laura Romero-Pérez; Ana Banito; Shunya Ohmura; Julian Musa; Thomas Kirchner Journal: Mol Cancer Date: 2022-01-03 Impact factor: 27.401
Authors: Iven Winklemann; Rei Matsuoka; Pascal F Meier; Denis Shutin; Chenou Zhang; Laura Orellana; Ricky Sexton; Michael Landreh; Carol V Robinson; Oliver Beckstein; David Drew Journal: EMBO J Date: 2020-10-29 Impact factor: 14.012