Qing Wang1, Xiaojie Lu2, Shidi Zhao2, Mingzhi Pang2, Xuechao Wu2, Heng Wu3, Robert M Hoffman4,5, Zhijian Yang3, Yan Zhang2. 1. Department of Neurosurgery, Neuroscience Center, Affiliated Wuxi Second Hospital of Nanjing Medical University, Wuxi, P.R. China wxwqnj@163.com. 2. Department of Neurosurgery, Neuroscience Center, Affiliated Wuxi Second Hospital of Nanjing Medical University, Wuxi, P.R. China. 3. Origin Biosciences Inc., Nanjing, P.R. China. 4. AntiCancer, Inc., San Diego, CA, U.S.A. 5. Department of Surgery, University of California, San Diego, CA, U.S.A.
Abstract
AIM: To investigate the effect of moesin expression on cell proliferaton and invasion of human glioblastoma cell lines in vitro. MATERIALS AND METHODS: Glioblastoma LN229 and U87 cells were transfected with the H4645-plenti-EGFP-moesin expression vector for moesin up-regulation. Moesin and β-catenin expression levels in the transfected cells were analyzed by real-time polymerase chain reaction (PCR) and Western blotting. Cell proliferation was measured using the CCK8 assay. Cell invasion and migration ability were assessed using a transwell cell invasion and wound-healing assay. RESULTS: Moesin mRNA and protein expression were significantly increased in the two transfected LN229-H4645 and U87-H4645 cell lines. β-catenin expression was increased by moesin up-regulation in the transfected LN229-H4645 and U87-H4645 cell lines. The CCK-8 assay revealed that up-regulating moesin results in a significant increase in glioblastoma cell proliferation. Glioblastoma cell invasion and migration were increased by moesin up-regulation. CONCLUSION: Up-regulation of moesin expression in glioblastoma cells correlated with increases in cell proliferation, invasion and migration, suggesting moesin's role in glioblastoma progression. Copyright
AIM: To investigate the effect of moesin expression on cell proliferaton and invasion of humanglioblastoma cell lines in vitro. MATERIALS AND METHODS:Glioblastoma LN229 and U87 cells were transfected with the H4645-plenti-EGFP-moesin expression vector for moesin up-regulation. Moesin and β-catenin expression levels in the transfected cells were analyzed by real-time polymerase chain reaction (PCR) and Western blotting. Cell proliferation was measured using the CCK8 assay. Cell invasion and migration ability were assessed using a transwell cell invasion and wound-healing assay. RESULTS:Moesin mRNA and protein expression were significantly increased in the two transfected LN229-H4645 and U87-H4645 cell lines. β-catenin expression was increased by moesin up-regulation in the transfected LN229-H4645 and U87-H4645 cell lines. The CCK-8 assay revealed that up-regulating moesin results in a significant increase in glioblastoma cell proliferation. Glioblastoma cell invasion and migration were increased by moesin up-regulation. CONCLUSION: Up-regulation of moesin expression in glioblastoma cells correlated with increases in cell proliferation, invasion and migration, suggesting moesin's role in glioblastoma progression. Copyright
Authors: Pinar Uysal-Onganer; Amy MacLatchy; Rayan Mahmoud; Igor Kraev; Paul R Thompson; Jameel M Inal; Sigrun Lange Journal: Int J Mol Sci Date: 2020-02-22 Impact factor: 5.923