Hsiang-Yu Tang1, Hung-Yao Ho2, Daniel Tsun-Yee Chiu3, Cheng-Yu Huang1, Mei-Ling Cheng4, Chiung-Mei Chen5. 1. Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Tao-yuan, Taiwan. 2. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-yuan, Taiwan; Clinical Phenome Center, Chang Gung Memorial Hospital, Tao-yuan, Taiwan. 3. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-yuan, Taiwan; Pediatric Hematology/Oncology, Chang Gung Memorial Hospital, Tao-yuan, Taiwan. 4. Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Tao-yuan, Taiwan; Clinical Phenome Center, Chang Gung Memorial Hospital, Tao-yuan, Taiwan; Department of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-yuan, Taiwan. Electronic address: chengm@mail.cgu.edu.tw. 5. Department of Neurology, Chang Gung Memorial Hospital, and College of Medicine, Chang Gung University, Tao-yuan, Taiwan. Electronic address: cmchen@adm.cgmh.org.tw.
Abstract
BACKGROUND: Guillain-Barré syndrome (GBS) is an acute inflammatory polyneuropathy resulting in demyelination in peripheral nervous system. Myelin enriched in lipids is easily oxidized by reactive oxygen species during inflammation. Oxidative stress and lipophilic anti-oxidative capacities in GBS patients have not been fully explored. To evaluate the redox status of GBS patients, we measured malondialdehyde (MDA), myeloperoxidase (MPO), lipophilic antioxidants, and tocopherols concentrations in plasma from GBS patients and age-matched healthy controls. RESULTS: Concentrations of γ-tocopherol and δ-tocopherol decreased significantly, and α-carotene significantly increased in GBS patients compared to healthy controls. However, no significant changes in MDA and MPO concentrations were detected. In GBS patients, the γ-tocopherol concentration correlated positively with concentrations of δ-tocopherol, α-tocopherol, lutein, Q10, and γ-CEHC, respectively. Similarly, the δ-tocopherol concentration correlated positively with γ-tocopherol, α-tocopherol, lutein, Q10, δ-CEHC, and γ-CEHC concentrations, respectively. The receiver operating characteristics curve analysis showed that γ-tocopherol may serve as a good predictor for GBS. CONCLUSIONS: Diminished lipophilic antioxidant defense, mainly γ-tocopherol and δ-tocopherol, in GBS patients accounting for their lowered resistance to reactive oxygen species is probably associated with pathogenesis of GBS, and potentially useful for the development of therapeutic strategies.
BACKGROUND: Guillain-Barré syndrome (GBS) is an acute inflammatory polyneuropathy resulting in demyelination in peripheral nervous system. Myelin enriched in lipids is easily oxidized by reactive oxygen species during inflammation. Oxidative stress and lipophilic anti-oxidative capacities in GBSpatients have not been fully explored. To evaluate the redox status of GBSpatients, we measured malondialdehyde (MDA), myeloperoxidase (MPO), lipophilic antioxidants, and tocopherols concentrations in plasma from GBSpatients and age-matched healthy controls. RESULTS: Concentrations of γ-tocopherol and δ-tocopherol decreased significantly, and α-carotene significantly increased in GBSpatients compared to healthy controls. However, no significant changes in MDA and MPO concentrations were detected. In GBSpatients, the γ-tocopherol concentration correlated positively with concentrations of δ-tocopherol, α-tocopherol, lutein, Q10, and γ-CEHC, respectively. Similarly, the δ-tocopherol concentration correlated positively with γ-tocopherol, α-tocopherol, lutein, Q10, δ-CEHC, and γ-CEHC concentrations, respectively. The receiver operating characteristics curve analysis showed that γ-tocopherol may serve as a good predictor for GBS. CONCLUSIONS: Diminished lipophilic antioxidant defense, mainly γ-tocopherol and δ-tocopherol, in GBSpatients accounting for their lowered resistance to reactive oxygen species is probably associated with pathogenesis of GBS, and potentially useful for the development of therapeutic strategies.