Transcriptional activation by the pseudorabies virus immediate early protein requires the TATA box element in the adenovirus 2 E1B promoter.

The adenovirus E1A protein and immediately early (IE) protein of pseudorabies virus stimulate transcription from many promoters. It has been postulated that these proteins function via cellular intermediates. We analyzed the transcription of adenovirus E1B promoter mutants in response to the stimulatory effect of IE protein in comparison with E1A. Far upstream deletions and precise Sp1 and TATA box substitution mutations were examined. Only mutations affecting the TATA box element interfered with transactivation by IE protein. This result is similar to observations for E1A transactivation (L. Wu et al. Nature (London) 326, 512-515, 1987), suggesting that both E1A and IE proteins mediate transactivation of the E1B promoter through a common cellular intermediate which interacts with the TATA box.