| Literature DB >> 28474118 |
Parameswaran Chidambaranathan1,2, Prasanth Tej Kumar Jagannadham1,2, Viswanathan Satheesh1,2, Deshika Kohli1, Santosh Halasabala Basavarajappa3, Bharadwaj Chellapilla3, Jitendra Kumar3, Pradeep Kumar Jain1,2, R Srinivasan4,5,6.
Abstract
The heat stress transcription factors (Hsfs) play a prominent role in thermotolerance and eliciting the heat stress response in plants. Identification and expression analysis of Hsfs gene family members in chickpea would provide valuable information on heat stress responsive Hsfs. A genome-wide analysis of Hsfs gene family resulted in the identification of 22 Hsf genes in chickpea in both desi and kabuli genome. Phylogenetic analysis distinctly separated 12 A, 9 B, and 1 C class Hsfs, respectively. An analysis of cis-regulatory elements in the upstream region of the genes identified many stress responsive elements such as heat stress elements (HSE), abscisic acid responsive element (ABRE) etc. In silico expression analysis showed nine and three Hsfs were also expressed in drought and salinity stresses, respectively. Q-PCR expression analysis of Hsfs under heat stress at pod development and at 15 days old seedling stage showed that CarHsfA2, A6, and B2 were significantly upregulated in both the stages of crop growth and other four Hsfs (CarHsfA2, A6a, A6c, B2a) showed early transcriptional upregulation for heat stress at seedling stage of chickpea. These subclasses of Hsfs identified in this study can be further evaluated as candidate genes in the characterization of heat stress response in chickpea.Entities:
Keywords: ABA-responsive elements (ABRE); DNA binding domain; Digital expression; Duplication of Hsfs; Heat stress responsive element (HSE); Heat stress-inducible gene expression; Phylogeny; Pod development stage
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Year: 2017 PMID： 28474118 DOI： 10.1007/s10265-017-0948-y
Source DB: PubMed Journal: J Plant Res ISSN： 0918-9440 Impact factor: 2.629