| Literature DB >> 28471459 |
Frederic Strobl1, Alexander Schmitz1, Ernst H K Stelzer1.
Abstract
Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition from on-demand to systematic data acquisition in developmental biology applications.Mesh:
Year: 2017 PMID: 28471459 DOI: 10.1038/nprot.2017.028
Source DB: PubMed Journal: Nat Protoc ISSN: 1750-2799 Impact factor: 13.491