Literature DB >> 2846641

DNA hybridization assay for congenital cytomegalovirus infection.

G J Buffone1, G J Demmler, C M Schimbor, M D Yow.   

Abstract

An in vitro DNA hybridization assay was used to test 281 newborns for congenital infection with cytomegalovirus. The assay utilized an abbreviated method for DNA preparation and a dot blot assay that provided good sensitivity (100%) and specificity (98.9%) when compared with standard tissue culture, yet substantially reduced the total time of analysis. This assay would be a useful adjunct to tissue culture to diagnose newborns with congenital infection with cytomegalovirus.

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Year:  1988        PMID： 2846641      PMCID： PMC266843          DOI： 10.1128/jcm.26.10.2184-2186.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Comparative study of diagnostic procedures for congenital cytomegalovirus infection.

Authors:  S Stagno; R F Pass; D W Reynolds; M A Moore; A J Nahmias; C A Alford
Journal:  Pediatrics       Date:  1980-02       Impact factor: 7.124

2.  Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1985-02       Impact factor: 5.948

3.  Detection of cytomegalovirus in urine samples by enzyme-linked immunosorbent assay.

Authors:  J A McKeating; S Stagno; P R Stirk; P D Griffiths
Journal:  J Med Virol       Date:  1985-08       Impact factor: 2.327

4.  Detection of cytomegalovirus in urine by nonisotopic DNA hybridization.

Authors:  G J Buffone; C M Schimbor; G J Demmler; D R Wilson; G J Darlington
Journal:  J Infect Dis       Date:  1986-07       Impact factor: 5.226

5.  Screening of newborn infants for cytomegalovirus infection.

Authors:  J G Starr; E Gold
Journal:  J Pediatr       Date:  1968-12       Impact factor: 4.406

6.  Spot-blot hybridization assay for the detection of hepatitis B virus DNA in serum: factors determining its sensitivity and specificity.

Authors:  E Walter; H E Blum; W B Offensperger; C Zeschnigk; S Offensperger; W Gerok
Journal:  Hepatology       Date:  1987 May-Jun       Impact factor: 17.425

7.  Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization.

Authors:  M Virtanen; A C Syvänen; J Oram; H Söderlund; M Ranki
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948

8.  Comparative serial virologic and serologic studies of symptomatic and subclinical congenitally and natally acquired cytomegalovirus infections.

Authors:  S Stagno; D W Reynolds; A Tsiantos; D A Fuccillo; W Long; C A Alford
Journal:  J Infect Dis       Date:  1975-11       Impact factor: 5.226

9.  Congenital cytomegalovirus infection: The relative importance of primary and recurrent maternal infection.

Authors:  S Stagno; R F Pass; M E Dworsky; R E Henderson; E G Moore; P D Walton; C A Alford
Journal:  N Engl J Med       Date:  1982-04-22       Impact factor: 91.245

10.  Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.

Authors:  S Chou; T C Merigan
Journal:  N Engl J Med       Date:  1983-04-21       Impact factor: 91.245
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