Jiaolin Zheng1, Dan Yi2, Xiaodong Shi1, Huaizhang Shi3. 1. Department of Neruology, The second hospital of Harbin Medical University, Harbin, Heilong Jiang, 150086, China. 2. Department of Pharmacology, Rush University Medical Center, Chicago, IL, 60607, USA. 3. Department of Neurosurgery, The first hospital of Harbin Medical University, Harbin, Heilong Jiang, 150001, China.
Abstract
OBJECTIVES: Neural stem cells (NSCs) are self-renewing, undifferentiated and multipotent precursors that can generate neuronal and glial lineages. MicroRNAs (miRNAs) are small non-coding RNAs that act crucial roles in cell proliferation, differentiation and migration. However, the role of miR-1297 in the development of NSCs is still unknown. MATERIALS AND METHODS: Primary NSCs were isolated from rat's embryos. The expression of miR-1297 and Hes1 were measured by qRT-PCR. Western blot was performed to detect the protein expression of Hes1, β-tubulin-III and GFAP. RESULTS: We showed that miR-1297 expression was upregulated during NSC differentiation, while the expression of Hes1 was decreased during NSC differentiation. Elevated expression of miR-1297 promoted the NSCs viability and increased the formation of NSCs to neurospheres. Ecoptic expression of miR-1297 promoted β-tubulin-III expression in the NSCs. Overexpression of miR-1297 decreased GFAP expression in the NSCs. Furthermore, we demonstrated that miR-1297 regulated NSCs viability and differentiation by directly targeting Hes1. Overexpression of miR-1297 suppressed Hes1 expression in the NSCs. CONCLUSIONS: These results suggested that miR-1297 played an important role in NSCs viability and differentiation through inhibiting Hes1 expression.
OBJECTIVES: Neural stem cells (NSCs) are self-renewing, undifferentiated and multipotent precursors that can generate neuronal and glial lineages. MicroRNAs (miRNAs) are small non-coding RNAs that act crucial roles in cell proliferation, differentiation and migration. However, the role of miR-1297 in the development of NSCs is still unknown. MATERIALS AND METHODS: Primary NSCs were isolated from rat's embryos. The expression of miR-1297 and Hes1 were measured by qRT-PCR. Western blot was performed to detect the protein expression of Hes1, β-tubulin-III and GFAP. RESULTS: We showed that miR-1297 expression was upregulated during NSC differentiation, while the expression of Hes1 was decreased during NSC differentiation. Elevated expression of miR-1297 promoted the NSCs viability and increased the formation of NSCs to neurospheres. Ecoptic expression of miR-1297 promoted β-tubulin-III expression in the NSCs. Overexpression of miR-1297 decreased GFAP expression in the NSCs. Furthermore, we demonstrated that miR-1297 regulated NSCs viability and differentiation by directly targeting Hes1. Overexpression of miR-1297 suppressed Hes1 expression in the NSCs. CONCLUSIONS: These results suggested that miR-1297 played an important role in NSCs viability and differentiation through inhibiting Hes1 expression.
Authors: Thomas Palm; Kathrin Hemmer; Julia Winter; Inga B Fricke; Katsiaryna Tarbashevich; Fereshteh Sadeghi Shakib; Ina-Maria Rudolph; Anna-Lena Hillje; Paola De Luca; Lamia'a Bahnassawy; Rabea Madel; Thomas Viel; Adriana De Siervi; Andreas H Jacobs; Sven Diederichs; Jens C Schwamborn Journal: Nucleic Acids Res Date: 2013-02-08 Impact factor: 16.971
Authors: Parker E Ludwig; Finosh G Thankam; Arun A Patil; Andrea J Chamczuk; Devendra K Agrawal Journal: Neural Regen Res Date: 2018-01 Impact factor: 5.135