| Literature DB >> 2846184 |
N von Mende1, D M Bird, P S Albert, D L Riddle.
Abstract
Mutations in the Caenorhabditis elegans dpy-13 (dumpy) gene result in a short, chunky body shape. This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. Three transposon insertion sites in dpy-13 are located near the 5' end of a 1.2 kb transcribed region. The EMS-induced reference allele, dpy-13(e184), carries a small deletion near the middle of this gene. The DNA sequence reveals that dpy-13 is a member of the collagen multi-gene family, and it could encode a polypeptide of 302 amino acids. A 146 base pair sequence, encoding amino acids 56-103, is unique in the C. elegans genome, and it hybridizes to a 1 kb mRNA of moderate abundance.Entities:
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Year: 1988 PMID： 2846184 DOI： 10.1016/0092-8674(88)90215-2
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582