Yael Shahal-Zimra1, Zohar Rotem1, Judith Chezar, Tzippy Shochat2, Liron Ross1, Itai Pickholtz1, Esther Rabizadeh1. 1. Hematology Laboratory, Felsenstein Medical Research Center, Rabin Medical Center (Beilinson Hospital), Petah Tikva, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. 2. Statistical Consultant, Rabin Medical Center (Beilinson Campus), Petah Tikva, Israel.
Abstract
BACKGROUND: Reference ranges for adult peripheral blood lymphocyte subsets have been established in a few countries. To the best of our knowledge no broad lymphocyte subset analysis of the Israeli population has been reported. Objectives: To establish reference ranges for healthy adults in Israel and to describe age- and gender-specific differences, if present. OBJECTIVES: To establish reference ranges for healthy adults in Israel and to describe age- and gender-specific differences, if present. METHODS: Lymphocyte subsets CD3, CD3/CD4, CD3/CD8, CD3-/CD16+/CD56+, CD3/TCRαβ, CD3/TCRγδ, and CD19 were examined by flow cytometry in 326 subjects. Samples were subdivided according to age and gender. RESULTS: Women of all ages had a significantly higher percentage and absolute counts of CD3/CD4 cells than their male counterparts. Higher CD3/CD4 cells were observed also in the older population (> 50 years). CD3/CD8 and CD3-/CD16+/CD56+ were higher in males. Older males had a lower total lymphocyte percentage and CD19 cells compared to younger men. No significant gender-related differences were observed in percent and number of CD19, CD3/TCRαβ or CD3/TCRγδ at all ages. CONCLUSIONS: These reference values could be useful in further studies for assessing changes that occur in different populations in human pathology.
BACKGROUND: Reference ranges for adult peripheral blood lymphocyte subsets have been established in a few countries. To the best of our knowledge no broad lymphocyte subset analysis of the Israeli population has been reported. Objectives: To establish reference ranges for healthy adults in Israel and to describe age- and gender-specific differences, if present. OBJECTIVES: To establish reference ranges for healthy adults in Israel and to describe age- and gender-specific differences, if present. METHODS: Lymphocyte subsets CD3, CD3/CD4, CD3/CD8, CD3-/CD16+/CD56+, CD3/TCRαβ, CD3/TCRγδ, and CD19 were examined by flow cytometry in 326 subjects. Samples were subdivided according to age and gender. RESULTS:Women of all ages had a significantly higher percentage and absolute counts of CD3/CD4 cells than their male counterparts. Higher CD3/CD4 cells were observed also in the older population (> 50 years). CD3/CD8 and CD3-/CD16+/CD56+ were higher in males. Older males had a lower total lymphocyte percentage and CD19 cells compared to younger men. No significant gender-related differences were observed in percent and number of CD19, CD3/TCRαβ or CD3/TCRγδ at all ages. CONCLUSIONS: These reference values could be useful in further studies for assessing changes that occur in different populations in human pathology.
Authors: Albert Font-Haro; Vaclav Janovec; Tomas Hofman; Ladislav Machala; David Jilich; Zora Melkova; Jan Weber; Katerina Trejbalova; Ivan Hirsch Journal: Viruses Date: 2018-03-28 Impact factor: 5.048
Authors: Anna M Kosyreva; Dzhuliia Sh Dzhalilova; Olga V Makarova; Ivan S Tsvetkov; Natalia A Zolotova; Marina A Diatroptova; Elena A Ponomarenko; Vladimir A Mkhitarov; Dmitriy N Khochanskiy; Liliya P Mikhailova Journal: Sci Rep Date: 2020-09-28 Impact factor: 4.379