| Literature DB >> 2845666 |
E Mendelson1, M G Smith, I L Miller, B J Carter.
Abstract
Adeno-associated virus (AAV) vectors readily express the gene for geneticin-resistance under control of the AAV p40 promoter when chromosomally integrated at low copy number in mammalian cells. We show that a truncated AAV rep gene, transcribed from the p5 and p19 promoters, mediates a negative effect on expression of geneticin-resistance in human 293 cells and a positive effect in HeLa cells. Also, we describe a novel phenotype for a mutant expressing the p19 rep gene alone which has a negative effect in 293 cells but no positive effect in HeLa cells.Entities:
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Year: 1988 PMID： 2845666 DOI： 10.1016/0042-6822(88)90536-3
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616