Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds.

The production of propolis by honeybees results from a selective collection of exudates from various plant species and present many potentialities in the pharmaceutical industry. The objective of this study was to investigate the chemical profile of Brazilian propolis, as well as their in vitro antioxidant and antibacterial activities. Gas chromatography-mass spectrometry was applied for chemical profiling of propolis extracts. Total phenolic compounds were quantified by Folin-Ciocalteu and antioxidant properties were assessed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Antibacterial activity was assessed against Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus. Correlation and multivariate statistical analysis were used to identify potential bioactive compounds in the extracts. Twenty-nine metabolites were identified along with 34 other metabolites that were classified into the following classes: triterpenoids (12), acetyltriterpenoids (3), sesquiterpenes (6), steroids (4), and hydrocarbons (9). The antioxidant capacity (IC50) ranged from 21.50 to 78.77μg/mL, whereas the content of total phenolic compounds ranged from 31.88 to 204.30mg GAE/g of dry weight. Total phenolic compounds and methyl retinoate showed a positive correlation with the antioxidant capacity, whereas tetradecanal, γ-palmitolactone and ethyl hydrocinnamate showed a negative correlation. Different sets of metabolites are shown to correlate with the antibacterial activity of the extracts, which is largely dependent on the type of microorganism. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying antibacterial activity encompass a complex trait, which might involve synergistic effects.