S Ksouri1, S Djebir2, A A Bentorki3, A Gouri3, Y Hadef3, A Benakhla4. 1. Department of Biology, Faculty of Nature and Life Sciences and Earth Sciences and the Universe, University of 8 Mai 1945 Guelma, BP 401, 24000 Guelma, Algeria. Electronic address: ksourisamir@gmail.com. 2. Department of Biology, Faculty of Nature and Life Sciences and Earth Sciences and the Universe, University of 8 Mai 1945 Guelma, BP 401, 24000 Guelma, Algeria. 3. Laboratory of Analytical Chemistry, Department of Pharmacy, Faculty of Medicine, University Bedji Mukhtar of Annaba, route Zaafrania, B.P. 205, 23000 Annaba, Algeria. 4. Institute of Veterinary Science, University Chadli Bendjedid El-Tarf, BP 73, 36000 El-Tarf, Algeria.
Abstract
OBJECTIVE: The aim of this study is to limit the antibiotic use in mastitis treatment and to find other alternatives. The antifungal activity of the essential oils from Origanum floribundum Munby., Rosmarinus officinalis L. and Thymus ciliatus Desf. is studied in the present work against a Candida albicans reference strain and ten C. albicans isolated strains from bovine clinical mastitis. MATERIALS AND METHODS: Essential oils were extracted by hydrodistillation technique using Clevenger apparatus. Their chromatographic analysis was performed with a Gas Chromatograph/Mass Spectrometer (GC/MS). Antifungal activities of essential oils were investigated by macrobroth method of dilution in tubes to determine the Minimum Inhibitory Concentrations (MIC 80%). RESULTS: Analysis of the essential oil showed chemical profile dominated by thymol (50.47 and 62.41%) and P-cymene (24.22 and 15.51%) in the oregano and the thyme respectively, 1, 8-cineol (31.50%) and α-pinene (18.33%) in Rosemary. The three essential oils revealed highly effective anticandidal activity, with an MIC of 80% values ranged from 15.02 to 31.08μg/mL. CONCLUSION: These results suggest that essential oils studied can be real alternatives in the control of mastitis fungi but deserving studies more in-depth and detailed on their application in vivo.
OBJECTIVE: The aim of this study is to limit the antibiotic use in mastitis treatment and to find other alternatives. The antifungal activity of the essential oils from Origanum floribundum Munby., Rosmarinus officinalis L. and Thymus ciliatus Desf. is studied in the present work against a Candidaalbicans reference strain and ten C. albicans isolated strains from bovine clinical mastitis. MATERIALS AND METHODS:Essential oils were extracted by hydrodistillation technique using Clevenger apparatus. Their chromatographic analysis was performed with a Gas Chromatograph/Mass Spectrometer (GC/MS). Antifungal activities of essential oils were investigated by macrobroth method of dilution in tubes to determine the Minimum Inhibitory Concentrations (MIC 80%). RESULTS: Analysis of the essential oil showed chemical profile dominated by thymol (50.47 and 62.41%) and P-cymene (24.22 and 15.51%) in the oregano and the thyme respectively, 1, 8-cineol (31.50%) and α-pinene (18.33%) in Rosemary. The three essential oils revealed highly effective anticandidal activity, with an MIC of 80% values ranged from 15.02 to 31.08μg/mL. CONCLUSION: These results suggest that essential oils studied can be real alternatives in the control of mastitis fungi but deserving studies more in-depth and detailed on their application in vivo.
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