Boram Choi1,2, Hyuk-Joon Lee1,3, Jimin Min1, Hwi-Nyeong Choe4, Yun-Suk Choi3, Young-Gil Son3, Hye-Seong Ahn3, Yun-Suhk Suh3, James R Goldenring5, Han-Kwang Yang1,3. 1. Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea. 2. Department of Life Science, Ewha Womans University, Seoul, Korea. 3. Department of Surgery. 4. Department of Nursing, Seoul National University Hospital, Seoul, Korea. 5. Department of Surgery, Vanderbilt University School of Medicine, Vanderbilt-Ingram Cancer Center, and the Nashville VA Medical Center, Nashville, TN, USA.
Abstract
BACKGROUND: Few reliable blood markers are available for detecting gastric cancer, mainly owing to the heterogeneity of the cancer. OBJECTIVE: To establish gastric cancer diagnostic markers, we evaluated the levels of plasma cadherin 17 (CDH17) and trefoil factor 3 (TFF3), which are secretory proteins and known markers for intestinal metaplasia (IM), in patients with gastric cancer. METHOD: The protein expression level was analyzed in blood plasma samples from 111 gastric cancer patients and 44 healthy individuals, using a sandwich ELISA kit, followed by statistical analyses. RESULT: Overall, the plasma levels of CDH17 and TFF3 were not significantly different between groups (p= 0.160 and p= 0.113, respectively). However, CDH17 expression was significantly elevated in patients with stage II and III gastric cancers compared to that in healthy controls (p= 0.023 and p= 0.037, respectively). In contrast, TFF3 levels were significantly elevated in patients with stage I (p= 0.001) and T1 gastric cancer (p= 0.013). The sensitivity and specificity of CDH17 were 66.7 and 61.4%, respectively (cutoff point: 0.189 ng/mL); for TFF3, these values were 62.2 and 56.8%, respectively (cutoff point: 5.215 ng/mL). CONCLUSIONS: These findings indicate that secretory protein markers for metaplastic lineages can be used as blood markers for gastric cancer.
BACKGROUND: Few reliable blood markers are available for detecting gastric cancer, mainly owing to the heterogeneity of the cancer. OBJECTIVE: To establish gastric cancer diagnostic markers, we evaluated the levels of plasma cadherin 17 (CDH17) and trefoil factor 3 (TFF3), which are secretory proteins and known markers for intestinal metaplasia (IM), in patients with gastric cancer. METHOD: The protein expression level was analyzed in blood plasma samples from 111 gastric cancerpatients and 44 healthy individuals, using a sandwich ELISA kit, followed by statistical analyses. RESULT: Overall, the plasma levels of CDH17 and TFF3 were not significantly different between groups (p= 0.160 and p= 0.113, respectively). However, CDH17 expression was significantly elevated in patients with stage II and III gastric cancers compared to that in healthy controls (p= 0.023 and p= 0.037, respectively). In contrast, TFF3 levels were significantly elevated in patients with stage I (p= 0.001) and T1 gastric cancer (p= 0.013). The sensitivity and specificity of CDH17 were 66.7 and 61.4%, respectively (cutoff point: 0.189 ng/mL); for TFF3, these values were 62.2 and 56.8%, respectively (cutoff point: 5.215 ng/mL). CONCLUSIONS: These findings indicate that secretory protein markers for metaplastic lineages can be used as blood markers for gastric cancer.
Entities:
Keywords:
CDH17; ELISA; TFF3; gastric cancer marker
Authors: Jonathan W J Lee; Feng Zhu; Supriya Srivastava; Stephen Kk Tsao; Christopher Khor; Khek Yu Ho; Kwong Ming Fock; Wee Chian Lim; Tiing Leong Ang; Wan Cheng Chow; Jimmy Bok Yan So; Calvin J Koh; Shijia Joy Chua; Andrew S Y Wong; Jaideepraj Rao; Lee Guan Lim; Khoon Lin Ling; Chung-King Chia; Choon Jin Ooi; Andrea Rajnakova; Wai Ming Yap; Manuel Salto-Tellez; Bow Ho; Richie Soong; Kee Seng Chia; Yik Ying Teo; Ming Teh; Khay-Guan Yeoh Journal: Gut Date: 2021-05-11 Impact factor: 23.059