Arnaud Sauer1, Ermanno Candolfi2, David Gaucher1, Catherine Creuzot-Garcher3, Alain Bron3, Christophe Chiquet4, Jean-Paul Berrod5, Nicolas Meyer6, Gilles Prevost7, Tristan Bourcier1. 1. a Fédération de Médecine Translationnelle, Service d'Ophtalmologie , Université de Strasbourg - CHU Strasbourg , Strasbourg , France. 2. b Institut de Parasitologie et de pathologies tropicales, Université de Strasbourg - CHU de Strasbourg , Strasbourg , France. 3. c Service d'Ophtalmologie , Centre Hospitalier Universitaire de Dijon , Dijon , France. 4. d Service d'Ophtalmologie , Centre Hospitalier Universitaire de Grenoble , Grenoble , France. 5. e Service d'Ophtalmologie , Centre Hospitalier Universitaire de Nancy , Nancy , France. 6. f Service de Méthodologie et Biostatistiques , Hôpitaux Universitaires de Strasbourg , BP 426 , Strasbourg , France. 7. g Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence bactérienne précoce, Institut de Bactériologie , Université de Strasbourg-CHRU , Strasbourg , France.
Abstract
PURPOSE: The host immune reaction during endophthalmitis, studied in particular through the intraocular cytokine network, is essential for the comprehension of the disease and the development of new therapies. Therefore, the purpose of this study was to elucidate the cytokine composition of aqueous humor during endophthalmitis. METHODS: In a multicenter case-control study, forty-nine patients with postoperative endophthalmitis and 60 controls (cataract surgery) were included. Visual acuity, local inflammatory grading, medical history and intraocular levels of 27 cytokines and chemokines (measured by multiplex immunoassay) were recorded. RESULTS: During endophtalmitis, an increase in total cytokines was observed. The raise of Th1 cytokines was particularly noticeable. Chemokines, such as IL-8, MIP-1 β, MCP-1, G-CSF and IP-10, also increased. Pearson's correlation analyses showed a poor visual prognosis with high levels of IL-8, MCP-1 and VEGF and a low level of IL-10 at admission. CONCLUSION: An increase in inflammatory cytokines is noticeable during endophthalmitis, with a particular emphasis on IL-8, MCP-1 and VEGF. Targeted anti-inflammatory and anti-VEGF treatments may be of interest in the future.
PURPOSE: The host immune reaction during endophthalmitis, studied in particular through the intraocular cytokine network, is essential for the comprehension of the disease and the development of new therapies. Therefore, the purpose of this study was to elucidate the cytokine composition of aqueous humor during endophthalmitis. METHODS: In a multicenter case-control study, forty-nine patients with postoperative endophthalmitis and 60 controls (cataract surgery) were included. Visual acuity, local inflammatory grading, medical history and intraocular levels of 27 cytokines and chemokines (measured by multiplex immunoassay) were recorded. RESULTS: During endophtalmitis, an increase in total cytokines was observed. The raise of Th1 cytokines was particularly noticeable. Chemokines, such as IL-8, MIP-1 β, MCP-1, G-CSF and IP-10, also increased. Pearson's correlation analyses showed a poor visual prognosis with high levels of IL-8, MCP-1 and VEGF and a low level of IL-10 at admission. CONCLUSION: An increase in inflammatory cytokines is noticeable during endophthalmitis, with a particular emphasis on IL-8, MCP-1 and VEGF. Targeted anti-inflammatory and anti-VEGF treatments may be of interest in the future.
Authors: Frederick C Miller; Phillip S Coburn; Mursalin Md Huzzatul; Austin L LaGrow; Erin Livingston; Michelle C Callegan Journal: Prog Retin Eye Res Date: 2019-05-28 Impact factor: 21.198