Draft Genome Sequence of MPKL 26, the Type Strain of the Novel Species Sinomonas mesophila.

Sinomonas mesophila MPKL 26T can produce silver nanoparticles. Here, we present the 4.0-Mb genome of this type strain, which contains 47 scaffolds with an N50 scaffold length of 261,266 bp. The availability of the genome sequence will provide a better understanding of strain MPKL 26T and the genus Sinomonas.

GENOME ANNOUNCEMENT

The genus Sinomonas was proposed by Zhou et al. (1) and comprises 10 species of Gram-positive actinobacteria (http://www.bacterio.net/sinomonas.html [2]). Members of the genus Sinomonas have the ability to produce silver nanoparticles (3), can hydrolyze starch (4), and are used for biodesulfurization coal (5). Here, we report the draft genome sequence of strain MPKL 26, the type strain of the novel species Sinomonas mesophila, which has the ability to produce silver nanoparticles (3).

Whole-genome sequencing of S. mesophila strain MPKL 26T was performed using a paired-end sequencing method with the HiSeq 2000 platform (Illumina, San Diego, CA, USA). Using 1.49 Gb of clean data, the draft genome was assembled with the SOAPdenovo2 package (6). The draft genome of S. mesophila MPKL 26T contains 47 scaffolds with a total size of 4,008,197 bp and with an N50 scaffold length of 261,266 bp. The G+C content is 71.1%. Gene prediction was carried out using Glimmer version 3.02 (7). The identification of tRNAs and rRNAs was carried out using tRNAscan-SE version 1.23 (8) and RNAmmer version 1.2 (9). A total of 3,742 genes were predicted with four rRNA and 52 tRNA genes. The predicted coding sequences were translated and used to search against the KEGG and COG databases. The functional annotation of the genome was also performed with RAST (10), which revealed 448 genes for carbohydrate metabolism, 235 genes involved in protein metabolism, and 90 genes involved in DNA metabolism. These data sources were combined to assert a product description for each predicted protein.

Accession number(s).

This whole-genome shotgun project has been deposited in DDBJ/EMBL/GenBank under the accession number MTIC00000000. The version described in this paper is the first version, MTIC01000000.