Maryam Pourhajibagher1, Nasim Chiniforush2, Sima Shahabi3, Shaghayegh Sobhani4, Mohammad Moein Monzavi4, Abbas Monzavi5, Abbas Bahador6. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Dental Implant Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran. 2. Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran. 3. Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran; Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 4. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 5. Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: ab.monzavi.laser@gmail.com. 6. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: abahador@tums.ac.ir.
Abstract
BACKGROUND: Aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. In this study, we described the outcome of the expression level of A. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) as a photosensitizing agent. MATERIALS AND METHODS: To determine the aPDT effect on the cell-surviving assay and expression ratio of the rcpA gene in A. actinomycetemcomitans by a colony-forming unit and relative quantitative (q) real-time PCR (qRT-PCR) assays, respectively, the proper dosing of sub-lethal aPDT was specified. RESULTS: The results of the current study showed that ICG-mediated aPDT, using 250-1000μg/mL, showed a significant reduction in A. actinomycetemcomitans growth when compared to the control group (P<0.05). Also, a sub-lethal dose of aPDT against A. actinomycetemcomitans was 125μg/mL ICG, with a 30s diode laser irradiation time at fluency of 15.6J/cm2 that could reduce the expression of rcpA gene approximately 6-fold. DISCUSSION: aPDT with ICG could reduce the cell survival and the virulence agent of A. actinomycetemcomitans. Thus, use of the appropriate aPDT dosage can be used for the successful treatment of periodontitis in vivo.
BACKGROUND:Aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. In this study, we described the outcome of the expression level of A. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) as a photosensitizing agent. MATERIALS AND METHODS: To determine the aPDT effect on the cell-surviving assay and expression ratio of the rcpA gene in A. actinomycetemcomitans by a colony-forming unit and relative quantitative (q) real-time PCR (qRT-PCR) assays, respectively, the proper dosing of sub-lethal aPDT was specified. RESULTS: The results of the current study showed that ICG-mediated aPDT, using 250-1000μg/mL, showed a significant reduction in A. actinomycetemcomitans growth when compared to the control group (P<0.05). Also, a sub-lethal dose of aPDT against A. actinomycetemcomitans was 125μg/mL ICG, with a 30s diode laser irradiation time at fluency of 15.6J/cm2 that could reduce the expression of rcpA gene approximately 6-fold. DISCUSSION: aPDT with ICG could reduce the cell survival and the virulence agent of A. actinomycetemcomitans. Thus, use of the appropriate aPDT dosage can be used for the successful treatment of periodontitis in vivo.