Literature DB >> 2845019

Omega-conotoxin GVIA binding to a high-affinity receptor in brain: characterization, calcium sensitivity, and solubilization.

J A Wagner1, A M Snowman, A Biswas, B M Olivera, S H Snyder.   

Abstract

We describe unique, high-affinity binding sites for omega[125I]conotoxin GVIA in membranes from rat brain and rabbit sympathetic ganglia which appear to be primarily associated with N-type voltage-dependent calcium channels. The dissociation constant (KD) for the toxin in rat brain membranes is 60 pM. Physiologic extracellular concentrations of calcium inhibit toxin binding noncompetitively (IC50 = 0.2 mM). The regional distribution of the binding sites in rat brain differs markedly from that of dihydropyridine calcium antagonist receptors associated with L-type calcium channels. In detergent-solubilized brain membranes, toxin binding retains the same affinity, specificity, and ionic sensitivity as in particulate preparations.

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Year:  1988        PMID： 2845019      PMCID： PMC6569423     

Source DB:  PubMed          Journal:  J Neurosci        ISSN： 0270-6474            Impact factor:   6.167


  23 in total

1.  Identification of CaV channel types expressed in muscle afferent neurons.

Authors:  Renuka Ramachandra; Bassil Hassan; Stephanie G McGrew; James Dompor; Mohamed Farrag; Victor Ruiz-Velasco; Keith S Elmslie
Journal:  J Neurophysiol       Date:  2013-07-10       Impact factor: 2.714

Review 2.  Metabolism and trafficking of N-type voltage-operated calcium channels in neurosecretory cells.

Authors:  E Sher; P Rosa; M Francolini; A Codignola; E Morlacchi; E Taverna; F Giovannini; A Brioschi; F Clementi; M W McEnery; M Passafaro
Journal:  J Bioenerg Biomembr       Date:  1998-08       Impact factor: 2.945

3.  N-Type calcium channels in the developing rat hippocampus: subunit, complex, and regional expression.

Authors:  O T Jones; G M Bernstein; E J Jones; D G Jugloff; M Law; W Wong; L R Mills
Journal:  J Neurosci       Date:  1997-08-15       Impact factor: 6.167

4.  Purified omega-conotoxin GVIA receptor of rat brain resembles a dihydropyridine-sensitive L-type calcium channel.

Authors:  M W McEnery; A M Snowman; A H Sharp; M E Adams; S H Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

5.  Inhibition of N-methyl-D-aspartate- and kainic acid-induced neurotransmitter release by omega-conotoxin GVIA.

Authors:  R A Keith; T J Mangano; A I Salama
Journal:  Br J Pharmacol       Date:  1989-11       Impact factor: 8.739

6.  Solution structure of the calcium channel antagonist omega-conotoxin GVIA.

Authors:  J J Skalicky; W J Metzler; D J Ciesla; A Galdes; A Pardi
Journal:  Protein Sci       Date:  1993-10       Impact factor: 6.725

7.  A selective N-type calcium channel antagonist protects against neuronal loss after global cerebral ischemia.

Authors:  K Valentino; R Newcomb; T Gadbois; T Singh; S Bowersox; S Bitner; A Justice; D Yamashiro; B B Hoffman; R Ciaranello
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-15       Impact factor: 11.205

8.  Actions of arginine polyamine on voltage and ligand-activated whole cell currents recorded from cultured neurones.

Authors:  R H Scott; M I Sweeney; E M Kobrinsky; H A Pearson; G H Timms; I A Pullar; S Wedley; A C Dolphin
Journal:  Br J Pharmacol       Date:  1992-05       Impact factor: 8.739

9.  Differential effects of calcium channel antagonists (omega-conotoxin GVIA, nifedipine, verapamil) on the electrically-evoked release of [3H]acetylcholine from the myenteric plexus, phrenic nerve and neocortex of rats.

Authors:  I Wessler; D J Dooley; J Werhand; F Schlemmer
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1990-04       Impact factor: 3.000

10.  Development of small molecules that mimic the binding of omega-conotoxins at the N-type voltage-gated calcium channel.

Authors:  Christina I Schroeder; Mark L Smythe; Richard J Lewis
Journal:  Mol Divers       Date:  2004       Impact factor: 2.943
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