Roland F Hoffmann1, Yuri M Moshkin2, Stijn Mouton1, Nicola A Grzeschik3, Ruby D Kalicharan3, Jeroen Kuipers3, Anouk H G Wolters3, Kazuki Nishida4, Aleksander V Romashchenko2,5, Jan Postberg6, Hans Lipps7, Eugene Berezikov1,5, Ody C M Sibon3, Ben N G Giepmans3, Peter M Lansdorp1,8. 1. European Research Institute for the Biology of Ageing, University of Groningen, University Medical Centre Groningen, A. Deusinglaan 1, NL-9713 AV Groningen, The Netherlands. 2. Department of Biochemistry, Erasmus University Medical Center, Dr. Molewaterplein 50, NL-3015 GE Rotterdam, The Netherlands. 3. Department of Cell Biology, University of Groningen, University Medical Centre Groningen, A. Deusinglaan 1, NL-9713 AV Groningen, The Netherlands. 4. Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan. 5. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia. 6. Helios Medical Centre Wuppertal, Paediatrics Centre, Witten/Herdecke University, Wuppertal, Germany. 7. Institute of Cell Biology, Centre for Biomedical Education and Research, Witten/Herdecke University, Witten, Germany. 8. Terry Fox Laboratory, British Columbia Cancer Agency and Department of Medicine, University of British Columbia Vancouver, BC, V5Z 1L3, Canada.
Nucleic Acids Res. (08 January 2016) 44 (1): 152–163. doi: 10.1093/nar/gkv900The corresponding Author and Editors wish to jointly express a note of concern regarding the above article.Recent studies by the corresponding Author and new collaborators have revealed that mouse monoclonal antibody 1H6, used in the above article to detect G-quadruplexes, cross-reacts with some other DNA sequences, notably adjacent thymidines in single stranded DNA that are restricted in their movement in G4 structures and denatured DNA fibers. While the data reported in the published article remain valid, the Editors and corresponding Author wish to alert Readers of this cross-reactivity as it is likely to affect the interpretation of the results of all experiments using the 1H6 antibody.The Editors commend the corresponding Author for being forthcoming and disclosing these latest results which have been also been published in NAR (1).Keith Fox, Senior Executive Editor, Nucleic Acids ResearchBarry Stoddard, Senior Executive Editor, Nucleic Acids Research