Zhengyi Cao1, Theodore Spilker2, Yongyi Fan1, Linda M Kalikin2, Susan Ciotti3, John J LiPuma2, Paul E Makidon1,4, John Erby Wilkinson4, James R Baker1,5, Su He Wang1,5. 1. Michigan Nanotechnology Institute for Medicine & Biological Sciences, University of Michigan, Ann Arbor, MI 48109, USA. 2. Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, MI 48109, USA. 3. NanoBio Corporation, Ann Arbor, MI 48105, USA. 4. Unit for Laboratory Animal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA. 5. Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
Abstract
AIM: To develop NB-201, a nanoemulsion compound, as a novel microbicidal agent against methicillin-resistant Staphylococcus aureus (MRSA) infection, which is a common threat to public health but with limited therapeutic options. MATERIALS & METHODS: NB-201 was tested in in vitro and in vivo murine and porcine models infected with MRSA. RESULTS: Topical treatment of MRSA-infected wounds with NB-201 significantly decreased bacterial load and had no toxic effects on healthy skin tissues. NB-201 attenuated neutrophil sequestration in MRSA-infected wounds and inhibited epidermal and deep dermal inflammation. The levels of proinflammatory cytokines were reduced in NB-201-treated MRSA-infected wounds. CONCLUSION: NB-201 can greatly reduce inflammation characteristic of infected wounds and has antimicrobial activity that effectively kills MRSA regardless of the genetic basis of antibiotic resistance.
AIM: To develop NB-201, a nanoemulsion compound, as a novel microbicidal agent against methicillin-resistant Staphylococcus aureus (MRSA) infection, which is a common threat to public health but with limited therapeutic options. MATERIALS & METHODS: NB-201 was tested in in vitro and in vivo murine and porcine models infected with MRSA. RESULTS: Topical treatment of MRSA-infected wounds with NB-201 significantly decreased bacterial load and had no toxic effects on healthy skin tissues. NB-201 attenuated neutrophil sequestration in MRSA-infected wounds and inhibited epidermal and deep dermal inflammation. The levels of proinflammatory cytokines were reduced in NB-201-treated MRSA-infected wounds. CONCLUSION: NB-201 can greatly reduce inflammation characteristic of infected wounds and has antimicrobial activity that effectively kills MRSA regardless of the genetic basis of antibiotic resistance.
Authors: Alexis Garcia; Yong Yi Fan; Sandeep Vellanki; Eun Young Huh; DiFernando Vanegas; Su He Wang; Soo Chan Lee Journal: mSphere Date: 2019-12-18 Impact factor: 4.389