Carina Muoth1, Mandy Großgarten2, Uwe Karst2, Jaime Ruiz3, Didier Astruc3, Sergio Moya4, Liliane Diener1, Kathrin Grieder5, Adrian Wichser1,6, Wolfram Jochum7, Peter Wick1, Tina Buerki-Thurnherr1. 1. Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. 2. Institute of Inorganic & Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 28/30, 48149 Münster, Germany. 3. ISM, Université de Bordeaux, 351 Cours de la Libération, 33405 Talence Cedex, France. 4. bCIC biomaGUNE, Unidad Biosuperficies, Paseo Miramon No. 182, Edif 'C' 20009 Donostia-San Sebastian, Spain. 5. Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. 6. Analytical Chemistry, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. 7. Institute of Pathology, Cantonal Hospital St. Gallen, Rorschacherstrasse 95, 9007 St. Gallen, Switzerland.
Abstract
AIM: Nanoparticle-based drug carriers hold great promise for the development of targeted therapies in pregnancy with reduced off-target effects. Here, we performed a mechanistic in vitro study on placental localization and penetration of gold nanoparticles (AuNPs) in dependence of particle size and surface modification. MATERIALS & METHODS: AuNP uptake and penetration in human placental coculture microtissues was assessed by inductively coupled plasma-mass spectrometry, transmission electron microscopy and laser ablation-inductively coupled plasma-mass spectrometry. RESULTS: Higher uptake and deeper penetration was observed for smaller (3-4 nm) or sodium carboxylate-modified AuNPs than for larger (13-14 nm) or PEGylate AuNPs, which barely passed the trophoblast barrier layer. CONCLUSION: It is possible to steer placental uptake and penetration of AuNPs by tailoring their properties, which is a prerequisite for the development of targeted therapies in pregnancy.
AIM: Nanoparticle-based drug carriers hold great promise for the development of targeted therapies in pregnancy with reduced off-target effects. Here, we performed a mechanistic in vitro study on placental localization and penetration of gold nanoparticles (AuNPs) in dependence of particle size and surface modification. MATERIALS & METHODS:AuNP uptake and penetration in human placental coculture microtissues was assessed by inductively coupled plasma-mass spectrometry, transmission electron microscopy and laser ablation-inductively coupled plasma-mass spectrometry. RESULTS: Higher uptake and deeper penetration was observed for smaller (3-4 nm) or sodium carboxylate-modified AuNPs than for larger (13-14 nm) or PEGylate AuNPs, which barely passed the trophoblast barrier layer. CONCLUSION: It is possible to steer placental uptake and penetration of AuNPs by tailoring their properties, which is a prerequisite for the development of targeted therapies in pregnancy.
Entities:
Keywords:
gold nanoparticles; placental coculture microtissues; uptake and penetration
Authors: Lea A Furer; Pietro Clement; Gordon Herwig; René M Rossi; Farien Bhoelan; Mario Amacker; Toon Stegmann; Tina Buerki-Thurnherr; Peter Wick Journal: Sci Rep Date: 2022-07-08 Impact factor: 4.996
Authors: Hanna M Dusza; Eugene A Katrukha; Sandra M Nijmeijer; Anna Akhmanova; A Dick Vethaak; Douglas I Walker; Juliette Legler Journal: Environ Health Perspect Date: 2022-09-21 Impact factor: 11.035