Yan Gu1, Yu-Jie Wu1, Qi Han1, Xi-Lian Zhou1, Chun Qiao1, Jian-Yong Li1, Zheng Ge2. 1. Department of Hematology, Jiangsu Province People's Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 2. Department of Hematology, Jiangsu Province People's Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China; Department of Hematology, Zhongda Hospital, Southeast University Medical School, Nanjing 210009, Jiangsu Province, China. E-mail: gezheng2008@163.com.
Abstract
OBJECTIVE: Cytokine receptor-like factor 2(CRLF2) plays an important role in the development of normal B lymphocytes, which can mediate early B cell proliferation and survival. The aim of this study was to investigate the mutations of CRLF2 and its clinical significance in adult patients with acute lymphoblastic leukemia(ALL). METHODS: Exons of CRLF2 were amplified, then the DNA was purified and sequenced; the frequency, position, types and clinical significance of CRLF2 mutations were analyzed. RESULTS: 6 types of genetic alterations in CRLF2 were found, among them the R186S prompted better prognosis, while L86I, F232F and W255C associated with poor prognosis. CONCLUSION: CRLF2 mutations may play an important role in the development and progressions of adult patients with ALL, and these genetic abnormalities may associate with clinical outcome.
OBJECTIVE: Cytokine receptor-like factor 2(CRLF2) plays an important role in the development of normal B lymphocytes, which can mediate early B cell proliferation and survival. The aim of this study was to investigate the mutations of CRLF2 and its clinical significance in adult patients with acute lymphoblastic leukemia(ALL). METHODS: Exons of CRLF2 were amplified, then the DNA was purified and sequenced; the frequency, position, types and clinical significance of CRLF2 mutations were analyzed. RESULTS: 6 types of genetic alterations in CRLF2 were found, among them the R186S prompted better prognosis, while L86I, F232F and W255C associated with poor prognosis. CONCLUSION:CRLF2 mutations may play an important role in the development and progressions of adult patients with ALL, and these genetic abnormalities may associate with clinical outcome.
Authors: H Chen; X J Wang; S Liu; F F Yuan; H Ai; L Chen; R H Mi; Y Y Xiong; M J Li; R H Fan; Q S Yin; X D Wei Journal: Zhonghua Xue Ye Xue Za Zhi Date: 2018-10-14