| Literature DB >> 2844179 |
K R Fox1.
Abstract
DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.Entities:
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Year: 1988 PMID: 2844179 DOI: 10.1016/s0006-291x(88)80563-1
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN: 0006-291X Impact factor: 3.575