Literature DB >> 2844179

DNAase I footprinting of restriction enzymes.

K R Fox1.   

Abstract

DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.

Entities:  

Mesh:

Substances:

Year:  1988        PMID: 2844179     DOI: 10.1016/s0006-291x(88)80563-1

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  6 in total

1.  Sequence-specific DNA binding by the MspI DNA methyltransferase.

Authors:  A K Dubey; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

2.  High resolution footprinting of EcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent.

Authors:  K Uchida; A M Pyle; T Morii; J K Barton
Journal:  Nucleic Acids Res       Date:  1989-12-25       Impact factor: 16.971

3.  High-Performance Image-Based Measurements of Biological Forces and Interactions in a Dual Optical Trap.

Authors:  Jessica L Killian; James T Inman; Michelle D Wang
Journal:  ACS Nano       Date:  2018-11-20       Impact factor: 15.881

4.  KpnI restriction endonuclease and methyltransferase exhibit contrasting mode of sequence recognition.

Authors:  Siddamadappa Chandrashekaran; U H Manjunatha; Valakunja Nagaraja
Journal:  Nucleic Acids Res       Date:  2004-06-10       Impact factor: 16.971

5.  Binding of MmeI restriction-modification enzyme to its specific recognition sequence is stimulated by S-adenosyl-L-methionine.

Authors:  Joanna Nakonieczna; Jaroslaw W Zmijewski; Bogdan Banecki; Anna J Podhajska
Journal:  Mol Biotechnol       Date:  2007-10       Impact factor: 2.695

6.  Single-pass transcription by T7 RNA polymerase.

Authors:  Luiz F M Passalacqua; Armine I Dingilian; Andrej Lupták
Journal:  RNA       Date:  2020-09-21       Impact factor: 4.942

  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.