Jacky Lautridou1, Vianney Pichereau2, Sébastien Artigaud2, Benoit Bernay3, Otto Barak4, Ryan Hoiland5, Andrew T Lovering6, Ingrid Eftedal7, Zeljko Dujic8, François Guerrero1. 1. Laboratoire ORPHY EA 4324, Université de Bretagne Occidentale, IBSAM, Breast, France. 2. LEMAR UMR 6539, Université de Bretagne Occidentale, CNRS/UBO/IRD/IFREMER, Breast, France. 3. Proteogen SF ICORE 4206, Université de Caen, Caen, France. 4. University of Novi Sad School of Medicine, Novi Sad, Serbia. 5. Okanagan Campus, University of British Columbia, Kelowna, British Columbia, Canada. 6. Department of Human Physiology, University of Oregon, Eugene, USA. 7. Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway. 8. Dept of Integrative Physiology, University of Split School of Medicine, Split, Croatia.
Abstract
PURPOSE: Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms. EXPERIMENTAL DESIGN: Twelve healthy male divers were subjected to a single dive at a depth of 18 m of sea water (msw) with a 47-min bottom time followed by a direct ascent to the surface at a rate of 9 msw/min. Venous blood was collected before the dive as well as 30 min and 2 h following the dive. The plasma proteomes from four individuals were then analyzed by using a two-dimensional electrophoresis-based proteomic strategy. RESULTS: No protein spot showed a significantly changed abundance (fdr< 0.1) between the tested times. CONCLUSION: These results strengthen the hypothesis according to which significant changes of the plasma proteome measurable with two-dimensional electrophoresis may only occur along with DCS symptoms.
PURPOSE: Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms. EXPERIMENTAL DESIGN: Twelve healthy male divers were subjected to a single dive at a depth of 18 m of sea water (msw) with a 47-min bottom time followed by a direct ascent to the surface at a rate of 9 msw/min. Venous blood was collected before the dive as well as 30 min and 2 h following the dive. The plasma proteomes from four individuals were then analyzed by using a two-dimensional electrophoresis-based proteomic strategy. RESULTS: No protein spot showed a significantly changed abundance (fdr< 0.1) between the tested times. CONCLUSION: These results strengthen the hypothesis according to which significant changes of the plasma proteome measurable with two-dimensional electrophoresis may only occur along with DCS symptoms.