Helmut Buhler1, Raphael Adamietz2, Theresa Abeln2, David Diaz-Carballo3, Pascaline Nguemgo-Kouam2, Thomas Hero4, Irenaus A Adamietz4. 1. Institute for Molecular Oncology, Radiobiology and Experimental Radiotherapy, Marien Hospital Herne, Medical Center Ruhr-University Bochum, Herne, Germany helmut.buehler@rub.de. 2. Institute for Molecular Oncology, Radiobiology and Experimental Radiotherapy, Marien Hospital Herne, Medical Center Ruhr-University Bochum, Herne, Germany. 3. Clinic for Hematology and Oncology, Medical Center Ruhr-University Bochum, Bochum, Germany. 4. Clinic for Radiotherapy and Radio-Oncology, Medical Center Ruhr-University Bochum, Bochum, Germany.
Abstract
AIM: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. MATERIALS AND METHODS: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with <1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. RESULTS: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. CONCLUSION: This study demonstrates that useful videographic systems can be constructed at low cost. Copyright
AIM: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. MATERIALS AND METHODS: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37°C). Points of interest can be revisited in definable intervals with <1 μm repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. RESULTS: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. CONCLUSION: This study demonstrates that useful videographic systems can be constructed at low cost. Copyright
Authors: C Ince; D L Ypey; M M Diesselhoff-Den Dulk; J A Visser; A De Vos; R Van Furth Journal: J Immunol Methods Date: 1983-05-27 Impact factor: 2.303