Hujun Cui1, Shengqiang Zhang2, Hongbo Zhou1, Ling Guo3. 1. Department of Oncology, Affiliated Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, 157011, China. 2. Department of Thoracic Surgery, Affiliated Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, 157011, China. 3. Department of Pathology, Affiliated Second Hospital, Mudanjiang Medical University, Mudanjiang, 157009, China. mdjguo1979@126.com.
Abstract
BACKGROUND: Esophageal squamous carcinoma (ESC) is one of the most fatal malignancies worldwide with increasing occurrences yet poor outcome. MicroRNAs were reported to play roles in ESC. AIMS: We aimed to understand how miRNAs affect the radiotherapy resistance of ESC. METHODS: MicroRNA assays, real-time PCR, and Western blot were performed for expression analysis of miR-93 and BTG3. Luciferase activity assay was conducted with mutated B-cell translocation gene 3 (BTG3) 3'-UTR sequence in the 3' end of luciferase sequence with miR-93 inhibitor. ESC cells were treated with irradiation (IR) and clonogenic assay was utilized to detect the cell viability. Human ESC xenograft mouse model was established and subjected to target IR treatment followed by tumor size analysis. RESULTS: MiR-93 was decreased and BTG3 was increased in ESC cells, with negative correlation of their expression in ESC tissues. MiR-93 directly targeted BTG3 3'-UTR by luciferase activity assay. Either miR-93 inhibition or BTG3 overexpression decreased radiation resistance. Furthermore, miR-93 inhibition suppressed radiation resistance through BTG3. CONCLUSIONS: Direct downregulation of BTG3 by miR-93 is able to render ESC resistant to radiotherapy, and both BTG3 and miR-93 may potentially serve as clinical markers for ESC and contribute to the treatment of ESC.
BACKGROUND:Esophageal squamous carcinoma (ESC) is one of the most fatal malignancies worldwide with increasing occurrences yet poor outcome. MicroRNAs were reported to play roles in ESC. AIMS: We aimed to understand how miRNAs affect the radiotherapy resistance of ESC. METHODS: MicroRNA assays, real-time PCR, and Western blot were performed for expression analysis of miR-93 and BTG3. Luciferase activity assay was conducted with mutated B-cell translocation gene 3 (BTG3) 3'-UTR sequence in the 3' end of luciferase sequence with miR-93 inhibitor. ESC cells were treated with irradiation (IR) and clonogenic assay was utilized to detect the cell viability. Human ESC xenograft mouse model was established and subjected to target IR treatment followed by tumor size analysis. RESULTS:MiR-93 was decreased and BTG3 was increased in ESC cells, with negative correlation of their expression in ESC tissues. MiR-93 directly targeted BTG3 3'-UTR by luciferase activity assay. Either miR-93 inhibition or BTG3 overexpression decreased radiation resistance. Furthermore, miR-93 inhibition suppressed radiation resistance through BTG3. CONCLUSIONS: Direct downregulation of BTG3 by miR-93 is able to render ESC resistant to radiotherapy, and both BTG3 and miR-93 may potentially serve as clinical markers for ESC and contribute to the treatment of ESC.
Authors: X L Ren; X H Zhu; X M Li; Y L Li; J M Wang; P X Wu; Z B Lv; W H Ma; W T Liao; W Wang; Y Q Ding; L Liang Journal: J Cancer Res Clin Oncol Date: 2014-09-20 Impact factor: 4.553
Authors: Ling Fang; William W Du; Weining Yang; Zina Jeyapalan Rutnam; Chun Peng; Haoran Li; Yunxia Q O'Malley; Ryan W Askeland; Sonia Sugg; Mingyao Liu; Tanvi Mehta; Zhaoqun Deng; Burton B Yang Journal: Cell Cycle Date: 2012-10-30 Impact factor: 4.534