Zhe Zhang1, Yuzhuo Yang1, Han Wu1, Hongliang Zhang2, Haitao Zhang1, Jiaming Mao3, Defeng Liu3, Lianming Zhao1, Haocheng Lin1, Wenhao Tang1, Kai Hong1, Hui Jiang4. 1. Department of Urology, Peking University Third Hospital, Beijing, 100191, China. 2. Department of Human Sperm Bank, Peking University Third Hospital, Beijing, 100191, China. 3. Department of Reproductive Medicine Center, Peking University Third Hospital, Beijing, 100191, China. 4. Department of Urology, Peking University Third Hospital, Beijing, 100191, China. jianghui55@163.com.
Abstract
PURPOSE: Sperm-specific sodium-hydrogen exchanger (sNHE) is essential to maintain sperm normal function in mice; however, its role in human sperm has not been clarified to date. The aim of this study is to investigate the expression pattern of sNHE in human spermatozoa and its relationship with sperm functional parameters. METHOD: Semen samples from 68 asthenozoospermic and 61 normozoospermic men were analyzed for sperm concentration, motility, and acrosome reaction, and high motile spermatozoa were collected by swim-up method. The expression of sNHE in spermatozoa was detected by Western blot and immunofluorescence staining. The relationship between sNHE expression and sperm parameters was assessed. RESULTS: We identified sNHE is mainly localized to the principal piece of the human sperm tail. The expression of sNHE was positively correlated with sperm concentration, total number, and progressive motility. Moreover, sNHE expression was upregulated in swim-up sperm and associated with most of sperm motility parameters including straight line velocity and curvilinear velocity. Our results also showed that sNHE expression is decreased in sperm from patients with asthenozoospermia compared with that from normal controls. However, no correlation was found between sNHE expression and acrosome reaction in spermatozoa. CONCLUSIONS: The expression pattern of sNHE suggested that this protein may be involved in the regulation of sperm motility, and aberration of its expression in sperm may contribute to the pathogenesis of asthenozoospermia.
PURPOSE: Sperm-specific sodium-hydrogen exchanger (sNHE) is essential to maintain sperm normal function in mice; however, its role in human sperm has not been clarified to date. The aim of this study is to investigate the expression pattern of sNHE in human spermatozoa and its relationship with sperm functional parameters. METHOD: Semen samples from 68 asthenozoospermic and 61 normozoospermic men were analyzed for sperm concentration, motility, and acrosome reaction, and high motile spermatozoa were collected by swim-up method. The expression of sNHE in spermatozoa was detected by Western blot and immunofluorescence staining. The relationship between sNHE expression and sperm parameters was assessed. RESULTS: We identified sNHE is mainly localized to the principal piece of the human sperm tail. The expression of sNHE was positively correlated with sperm concentration, total number, and progressive motility. Moreover, sNHE expression was upregulated in swim-up sperm and associated with most of sperm motility parameters including straight line velocity and curvilinear velocity. Our results also showed that sNHE expression is decreased in sperm from patients with asthenozoospermia compared with that from normal controls. However, no correlation was found between sNHE expression and acrosome reaction in spermatozoa. CONCLUSIONS: The expression pattern of sNHE suggested that this protein may be involved in the regulation of sperm motility, and aberration of its expression in sperm may contribute to the pathogenesis of asthenozoospermia.
Entities:
Keywords:
Asthenozoospermia; Human sperm; Male infertility; Motility parameters; Sodium-hydrogen exchanger
Authors: Rubén D Peralta-Arias; Carmen Y Vívenes; María I Camejo; Sandy Piñero; Teresa Proverbio; Elizabeth Martínez; Reinaldo Marín; Fulgencio Proverbio Journal: Reproduction Date: 2015-05 Impact factor: 3.906
Authors: Dan Wang; Jie Hu; I Alexandru Bobulescu; Timothy A Quill; Paul McLeroy; Orson W Moe; David L Garbers Journal: Proc Natl Acad Sci U S A Date: 2007-05-17 Impact factor: 11.205
Authors: Takuya Nishigaki; Omar José; Ana Laura González-Cota; Francisco Romero; Claudia L Treviño; Alberto Darszon Journal: Biochem Biophys Res Commun Date: 2014-06-02 Impact factor: 3.575
Authors: Leila Adamyan; Vladimir Elagin; Valeriy Vechorko; Assia Stepanian; Anton Dashko; Dmitriy Doroshenko; Yana Aznaurova; Maxim Sorokin; Maria Suntsova; Alexei Drobyshev; Andrew Garazha; Anton Buzdin Journal: F S Sci Date: 2021-08-05
Authors: Priyanka Prajapati; Shruti Kane; Rachel C McBrinn; Morven S Dean; Sarah J Martins da Silva; Sean G Brown Journal: Int J Mol Sci Date: 2022-09-24 Impact factor: 6.208
Authors: Lis C Puga Molina; Guillermina M Luque; Paula A Balestrini; Clara I Marín-Briggiler; Ana Romarowski; Mariano G Buffone Journal: Front Cell Dev Biol Date: 2018-07-27