Literature DB >> 28432219

Optimized CUBIC protocol for three-dimensional imaging of chicken embryos at single-cell resolution.

María Victoria Gómez-Gaviro1,2,3, Evan Balaban4, Diana Bocancea5, María Teresa Lorrio5, Maria Pompeiano4, Manuel Desco5,2,3, Jorge Ripoll5,2, Juan José Vaquero5,2.   

Abstract

The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.
© 2017. Published by The Company of Biologists Ltd.

Entities:  

Keywords:  3D imaging; Chicken embryo; Light-sheet microscopy; Tissue clearing

Mesh：

Substances：

Year:  2017        PMID： 28432219     DOI： 10.1242/dev.145805

Source DB:  PubMed          Journal:  Development        ISSN： 0950-1991            Impact factor:   6.868


  10 in total

1.  Imaging the brain in 3D using a combination of CUBIC and immunofluorescence staining.

Authors:  Yangyang Xu; Peng Li; Mengqi Wang; Jie Zhang; Wei Wang
Journal:  Biomed Opt Express       Date:  2019-04-01       Impact factor: 3.732

2.  Looking inside the heart: a see-through view of the vascular tree.

Authors:  Imke Nehrhoff; Jorge Ripoll; Rafael Samaniego; Manuel Desco; Maria Victoria Gómez-Gaviro
Journal:  Biomed Opt Express       Date:  2017-05-30       Impact factor: 3.732

3.  A functional circuit formed by the autonomic nerves and myofibroblasts controls mammalian alveolar formation for gas exchange.

Authors:  Kuan Zhang; Erica Yao; Shao-An Wang; Ethan Chuang; Julia Wong; Liliana Minichiello; Andrew Schroeder; Walter Eckalbar; Paul J Wolters; Pao-Tien Chuang
Journal:  Dev Cell       Date:  2022-06-16       Impact factor: 13.417

4.  Double-layered two-directional somatopleural cell migration during chicken body wall development revealed with local fluorescent tissue labeling.

Authors:  Nobuyuki Sakamoto; Hirohiko Aoyama; Koji Ikegami
Journal:  Anat Sci Int       Date:  2022-02-20       Impact factor: 1.693

5.  In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Authors:  Jiyoung Lee; Akito Sutani; Rin Kaneko; Jun Takeuchi; Tetsuo Sasano; Takashi Kohda; Kensuke Ihara; Kentaro Takahashi; Masahiro Yamazoe; Tomohiro Morio; Tetsushi Furukawa; Fumitoshi Ishino
Journal:  Nat Commun       Date:  2020-09-03       Impact factor: 14.919

Review 6.  Tissue clearing and its applications in neuroscience.

Authors:  Hiroki R Ueda; Ali Ertürk; Kwanghun Chung; Viviana Gradinaru; Alain Chédotal; Pavel Tomancak; Philipp J Keller
Journal:  Nat Rev Neurosci       Date:  2020-02       Impact factor: 34.870

7.  Tissue Clearing and Light Sheet Microscopy: Imaging the Unsectioned Adult Zebra Finch Brain at Cellular Resolution.

Authors:  Mariana Diales Rocha; Daniel Normen Düring; Philipp Bethge; Fabian F Voigt; Staffan Hildebrand; Fritjof Helmchen; Alexander Pfeifer; Richard Hans Robert Hahnloser; Manfred Gahr
Journal:  Front Neuroanat       Date:  2019-02-14       Impact factor: 3.856

8.  Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids.

Authors:  Dwaipayan Adhya; George Chennell; James A Crowe; Eva P Valencia-Alarcón; James Seyforth; Neveen A Hosny; Marina V Yasvoina; Robert Forster; Simon Baron-Cohen; Anthony C Vernon; Deepak P Srivastava
Journal:  Mol Autism       Date:  2021-01-22       Impact factor: 7.509

9.  A 3D Analysis of Cleared Human Melanoma.

Authors:  Vicente Llorente; Daniel Sanderson; Alejandro Martín-Gorgojo; Rafael Samaniego; Manuel Desco; María Victoria Gómez-Gaviro
Journal:  Biomedicines       Date:  2022-07-02

10.  Investigation of PRDM10 and PRDM13 Expression in Developing Mouse Embryos by an Optimized PACT-Based Embryo Clearing Method.

Authors:  Jiwon Woo; Byung-Ho Jin; Mirae Lee; Eunice Yoojin Lee; Hyung-Seok Moon; Jeong-Yoon Park; Yong-Eun Cho
Journal:  Int J Mol Sci       Date:  2021-03-12       Impact factor: 5.923
  10 in total

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